Immobilization and condensation of DNA with 3-aminopropyltriethoxysilane studied by atomic force microscopy

被引:36
作者
Liu, Z [1 ]
Li, Z [1 ]
Zhou, H [1 ]
Wei, G [1 ]
Song, Y [1 ]
Wang, L [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
关键词
AFM; 3-aminopropyltriethoxysilane; APTES; atomic force microscopy; condensation; DNA; mica;
D O I
10.1111/j.1365-2818.2005.01482.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
We used different methods to modify a mica surface with 3-aminopropyltriethoxysilane (APTES), and then used it as substrate to immobilize DNA for atomic force microscopy (AFM) observation. The evaporation method and solution modifying method were investigated and evaluated. The solution modifying method was found to be relatively simple and effective. Using an APTES solution-modified mica surface, DNA immobilization appeared more reproducible and it could be imaged in liquid. The mixed solution of APTES and DNA was dropped directly onto the mica surface for AFM imaging. We found that DNA can condense in APTES water solutions. Toroids, rods and intermediate structures of condensation were captured by AFM.
引用
收藏
页码:233 / 239
页数:7
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