Internalization of Flax Rust Avirulence Proteins into Flax and Tobacco Cells Can Occur in the Absence of the Pathogen

被引:134
作者
Rafiqi, Maryam [2 ]
Gan, Pamela H. P. [2 ]
Ravensdale, Michael [1 ]
Lawrence, Gregory J. [1 ]
Ellis, Jeffrey G. [1 ]
Jones, David A. [2 ]
Hardham, Adrienne R. [2 ]
Dodds, Peter N. [1 ]
机构
[1] CSIRO Plant Ind, Canberra, ACT 2601, Australia
[2] Australian Natl Univ, Coll Med Biol & Environm, Res Sch Biol, Div Plant Sci, Canberra, ACT 0200, Australia
基金
澳大利亚研究理事会; 美国国家卫生研究院;
关键词
HOST-TARGETING SIGNAL; PLANT-CELLS; PENETRATING PEPTIDES; CELLULAR UPTAKE; PHYTOPHTHORA-INFESTANS; RESISTANCE PROTEIN; DISEASE RESISTANCE; SECRETED PROTEINS; EFFECTOR PROTEINS; MALARIA PARASITES;
D O I
10.1105/tpc.109.072983
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Translocation of pathogen effector proteins into the host cell cytoplasm is a key determinant for the pathogenicity of many bacterial and oomycete plant pathogens. A number of secreted fungal avirulence (Avr) proteins are also inferred to be delivered into host cells, based on their intracellular recognition by host resistance proteins, including those of flax rust (Melampsora lini). Here, we show by immunolocalization that the flax rust AvrM protein is secreted from haustoria during infection and accumulates in the haustorial wall. Five days after inoculation, the AvrM protein was also detected within the cytoplasm of a proportion of plant cells containing haustoria, confirming its delivery into host cells during infection. Transient expression of secreted AvrL567 and AvrM proteins fused to cerulean fluorescent protein in tobacco (Nicotiana tabacum) and flax cells resulted in intracellular accumulation of the fusion proteins. The rust Avr protein signal peptides were functional in plants and efficiently directed fused cerulean into the secretory pathway. Thus, these secreted effectors are internalized into the plant cell cytosol in the absence of the pathogen, suggesting that they do not require a pathogen-encoded transport mechanism. Uptake of these proteins is dependent on signals in their N-terminal regions, but the primary sequence features of these uptake regions are not conserved between different rust effectors.
引用
收藏
页码:2017 / 2032
页数:16
相关论文
共 57 条
[11]   The AvrM Effector from Flax Rust Has a Structured C-Terminal Domain and Interacts Directly with the M Resistance Protein [J].
Catanzariti, Ann-Maree ;
Dodds, Peter N. ;
Ve, Thomas ;
Kobe, Bostjan ;
Ellis, Jeffrey G. ;
Staskawicz, Brian J. .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2010, 23 (01) :49-57
[12]   N-terminal processing of proteins exported by malaria parasites [J].
Chang, Henry H. ;
Falick, Arnold M. ;
Carlton, Peter M. ;
Sedat, John W. ;
DeRisi, Joseph L. ;
Marletta, Michael A. .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2008, 160 (02) :107-115
[13]   Host-microbe interactions: Shaping the evolution of the plant immune response [J].
Chisholm, ST ;
Coaker, G ;
Day, B ;
Staskawicz, BJ .
CELL, 2006, 124 (04) :803-814
[14]   Cellular uptake of cell-penetrating peptides pVEC and transportan in plants [J].
Chugh, Archana ;
Eudes, Francois .
JOURNAL OF PEPTIDE SCIENCE, 2008, 14 (04) :477-481
[15]   A newly discovered protein export machine in malaria parasites [J].
de Koning-Ward, Tania F. ;
Gilson, Paul R. ;
Boddey, Justin A. ;
Rug, Melanie ;
Smith, Brian J. ;
Papenfuss, Anthony T. ;
Sanders, Paul R. ;
Lundie, Rachel J. ;
Maier, Alexander G. ;
Cowman, Alan F. ;
Crabb, Brendan S. .
NATURE, 2009, 459 (7249) :945-U66
[16]   EXPRESSION OF FOREIGN GENES IN REGENERATED PLANTS AND IN THEIR PROGENY [J].
DEBLOCK, M ;
HERRERAESTRELLA, L ;
VANMONTAGU, M ;
SCHELL, J ;
ZAMBRYSKI, P .
EMBO JOURNAL, 1984, 3 (08) :1681-1689
[17]   PLANT AND MAMMALIAN SORTING SIGNALS FOR PROTEIN RETENTION IN THE ENDOPLASMIC-RETICULUM CONTAIN A CONSERVED EPITOPE [J].
DENECKE, J ;
DERYCKE, R ;
BOTTERMAN, J .
EMBO JOURNAL, 1992, 11 (06) :2345-2355
[18]  
DEROSSI D, 1994, J BIOL CHEM, V269, P10444
[19]   Direct protein interaction underlies gene-for-gene specificity and coevolution of the flax resistance genes and flax rust avirulence genes [J].
Dodds, Peter N. ;
Lawrence, Gregory J. ;
Catanzariti, Ann-Maree ;
Teh, Trazel ;
Wang, Ching-I. A. ;
Ayliffe, Michael A. ;
Kobe, Bostjan ;
Ellis, Jeffrey G. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (23) :8888-8893
[20]   The Melampsora lini AvrL567 avirulence genes are expressed in haustoria and their products are recognized inside plant cells [J].
Dodds, PN ;
Lawrence, GJ ;
Catanzariti, AM ;
Ayliffe, MA ;
Ellis, JG .
PLANT CELL, 2004, 16 (03) :755-768