A colorimetric biosensor for the detection of foodborne bacteria

被引：46

作者：

dos Santos Pires, Ana Clarissa ^{[1
]}

Ferreira Soares, Nilda de Fatima ^{[1
]}

Mendes da Silva, Luis Henrique ^{[2
]}

Hespanhol da Silva, Maria do Carmo ^{[2
]}

De Almeida, Mauro Vieira ^{[3
]}

Le Hyaric, Mireille ^{[3
]}

de Andrade, Nelio Jose ^{[1
]}

Soares, Remili Freitas ^{[1
]}

Mageste, Aparecida Barbosa ^{[2
]}

Reis, Samira Gama ^{[3
]}

机构：

[1] Univ Fed Vicosa, Dept Tecnol Alimentos, BR-36570000 Vicosa, MG, Brazil

[2] Univ Fed Vicosa, Dept Quim, BR-36570000 Vicosa, MG, Brazil

[3] Univ Fed Juiz de Fora, Dept Quim, BR-36036330 Juiz De Fora, Brazil

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2011年 / 153卷 / 01期

关键词：

Polydiacetylene; Biosensor; Bacteria; Food microbiology; ESCHERICHIA-COLI; POLYDIACETYLENE; TRANSITIONS; RECEPTOR; CHROMISM;

D O I：

10.1016/j.snb.2010.09.069

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have synthesized 10,12-pentacosadyinoic acid (PCDA) + N-[(2-tetradecanamide)-ethyl]-ribonamide (TDER) vesicles to determine the colorimetric response induced by pathogenic bacteria (Staphylococcus aureus and Escherichia colt). The addition of bacterial supernatants caused a colorimetric transition in TDER/PCDA vesicles, even in diluted concentrations, indicating that chemical interactions occur between the vesicles and bacterial compounds. Bacterial substrates released from S. aureus induced a greater color change compared to the color change induced by E. colt. S. aureus metabolites also induced a greater color change when TDER/PCDA vesicles were incorporated into the cellulose strips. We determined the energy involved in the interaction between the bacterial substrates and the TDER/PCDA vesicles to be exothermic (and very high) for both bacterial supernatants. In addition, we analyzed the colorimetric transition in the presence of other molecules, using apple juice as a food matrix. Both apple juice and apple juice inoculated with bacterial substrates induced a TDER/PCDA color change; however, the S. aureus supernatant induced a slightly greater color change in the vesicles both in the suspension and in the cellulose strips. TDER/PCDA vesicles show great potential to be used as a biosensor to detect food pathogens in intelligent food packaging. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：17 / 23

页数：7

共 29 条

[1] Potential for detection of microorganisms and heavy metals in potable water using electronic nose technology [J].

Canhoto, OF ;

Magan, N .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (5-6) :751-754

[2] Polydiacetylene films: a review of recent investigations into chromogenic transitions and nanomechanical properties [J].

Carpick, RW ;

Sasaki, DY ;

Marcus, MS ;

Eriksson, MA ;

Burns, AR .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2004, 16 (23) :R679-R697

[3]

CHAMPAIBOON T, 2009, SENSOR ACTUAT B-CHEM, P532

[4] CHROMISM IN POLYDIACETYLENE SOLUTIONS AND CRYSTALS [J].

CHANCE, RR .

MACROMOLECULES, 1980, 13 (02) :396-398

[5] DIRECT COLORIMETRIC DETECTION OF A RECEPTOR-LIGAND INTERACTION BY A POLYMERIZED BILAYER ASSEMBLY [J].

CHARYCH, DH ;

NAGY, JO ;

SPEVAK, W ;

BEDNARSKI, MD .

SCIENCE, 1993, 261 (5121) :585-588

[6] Construction of Effective Receptor for Recognition of Avian Influenza H5N1 Protein HA1 by Assembly of Monohead Glycolipids on Polydiacetylene Vesicle Surface [J].

Deng, Jieli ;

Sheng, Zhonghan ;

Zhou, Kai ;

Duan, Mingxing ;

Yu, Chu-yi ;

Jiang, Long .

BIOCONJUGATE CHEMISTRY, 2009, 20 (03) :533-537

[7] Polydiacetylene as a Biosensor: Fundamentals and Applications in the Food Industry [J].

dos Santos Pires, Ana Clarissa ;

Ferreira Soares, Nilda de Fatima ;

Mendes da Silva, Luis Henrique ;

de Andrade, Nelio Jose ;

Araujo Silveira, Miriam Fontes ;

de Carvalho, Antonio Fernandes .

FOOD AND BIOPROCESS TECHNOLOGY, 2010, 3 (02) :172-181

[8] Micromechanical oscillators as rapid biosensor for the detection of active growth of Escherichia coli [J].

Gfeller, KY ;

Nugaeva, N ;

Hegner, M .

BIOSENSORS & BIOELECTRONICS, 2005, 21 (03) :528-533

[9] Investigation on the temperature effect of a mixed vesicle composed of polydiacetylene and BODIPY 558 [J].

Guo, Caixin ;

Zhang, Renzhe ;

Jiang, Long ;

Liu, Tao .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2007, 60 (01) :41-45

[10]

*I A LUTZ, 2005, NORM AN MET QUIM FIS

← 1 2 3 →