Clickable Enzyme-Linked Immunosorbent Assay

被引：22

作者：

Canalle, Luiz A. ^{[2
]}

Vong, TuHa ^{[2
]}

Adams, P. Hans H. M. ^{[2
]}

van Delft, Floris L. ^{[2
]}

Raats, Jos M. H. ^{[1
]}

Chirivi, Renato G. S. ^{[1
]}

van Hest, Jan C. M. ^{[2
]}

机构：

[1] ModiQuest Res BV, NL-6525 AJ Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Inst Mol & Mat, NL-6525 AJ Nijmegen, Netherlands

来源：

BIOMACROMOLECULES | 2011年 / 12卷 / 10期

关键词：

PHASE PEPTIDE-SYNTHESIS; LABILE ANCHOR GROUPS; COPPER-FREE; LIVING CELLS; CHEMISTRY; SURFACES; IMMOBILIZATION; SPECIFICITY; SENSITIVITY; COATINGS;

D O I：

10.1021/bm2009137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Click chemistry is explored as a potential cost- effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing either a terminal alkyne or a bicycle[6.19]non-4-yne (BCN) chemical handle, and a diagnositc peptide was subsequently immobilized onto these coatings by the copper-catalyzed azide-alkyne 1,3-dipolar cycloaddition (CuAAC) or copper-free strain- promoted azide-alkyne 1,3-dipolar cycloaddition (SPAAC) respectively. The terminal alkyne-containing coating showed high background levels in subsequent. ELISA's due to the copper catalyst used in the immobilization step. The BCN containing coating however, was successfully employed and presents a cost-effective alternative to existing (strept) avidin-biotin immobolization methods. This technology was illustrated with an ELISA used for the diagnosis of heumatoid arthritis (RA) but could be easily applied to wide range of diagnostic tests.

引用

页码：3692 / 3697

页数：6

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