A reagentless biosensing assembly based on quantum dot-donor Forster resonance energy transfer

被引：101

作者：

Medintz, IL

Clapp, AR

Melinger, JS

Deschamps, JR

Mattoussi, H

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA

[3] USN, Res Lab, Div Elect Sci & Technol, Washington, DC 20375 USA

[4] USN, Res Lab, Struct Matter Lab, Washington, DC 20375 USA

来源：

ADVANCED MATERIALS | 2005年 / 17卷 / 20期

关键词：

D O I：

10.1002/adma.200500722

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

On the surface of a quantum dot (QD), maltose-binding protein (MBP, white and yellow in Figure) labeled with a cyanine dye (red) self-assembles, resulting in Forster resonance energy transfer between the acceptor dye and the OD donor. Upon binding maltose, MBP undergoes a conformational change (white reversible arrow yellow), causing concentration-dependent photoluminescence changes.

引用

页码：2450 / +

页数：7

共 25 条

[1] Quantum dots as photosensitizers?
Bakalova, R
Ohba, H
Zhelev, Z
Ishikawa, M
Baba, Y
[J]. NATURE BIOTECHNOLOGY, 2004, 22 (11) : 1360 - 1361
[2] A fluorescence affinity hollow fiber sensor for continuous transdermal glucose monitoring
Ballerstadt, R
Schultz, JS
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (17) : 4185 - 4192
[3] Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins
Benson, DE
Conrad, DW
de Lorimer, RM
Trammell, SA
Hellinga, HW
[J]. SCIENCE, 2001, 293 (5535) : 1641 - 1644
[4] Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors
Clapp, AR
Medintz, IL
Mauro, JM
Fisher, BR
Bawendi, MG
Mattoussi, H
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) : 301 - 310
[5] Construction of a fluorescent biosensor family
De Lorimier, RM
Smith, JJ
Dwyer, MA
Looger, LL
Sali, KM
Paavola, CD
Rizk, SS
Sadigov, S
Conrad, DW
Loew, L
Hellinga, HW
[J]. PROTEIN SCIENCE, 2002, 11 (11) : 2655 - 2675
[6] Visualization of maltose uptake in living yeast cells by fluorescent nanosensors
Fehr, M
Frommer, WB
Lalonde, S
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (15) : 9846 - 9851
[7] ENGINEERING THE MALTOSE-BINDING PROTEIN FOR REAGENTLESS FLUORESCENCE SENSING
GILARDI, G
ZHOU, LQ
HIBBERT, L
CASS, AEG
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (21) : 3840 - 3847
[8] A hybrid quantum dot-antibody fragment fluorescence resonance energy transfer-based TNT sensor
Goldman, ER
Medintz, IL
Whitley, JL
Hayhurst, A
Clapp, AR
Uyeda, HT
Deschamps, JR
Lassman, ME
Mattoussi, H
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (18) : 6744 - 6751
[9] Adaptation of inorganic quantum dots for stable molecular beacons
Kim, JH
Morikis, D
Ozkan, M
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2004, 102 (02): : 315 - 319
[10] Computational design of receptor and sensor proteins with novel functions
Looger, LL
Dwyer, MA
Smith, JJ
Hellinga, HW
[J]. NATURE, 2003, 423 (6936) : 185 - 190

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