A reagentless biosensing assembly based on quantum dot-donor Forster resonance energy transfer

被引：101

作者：

Medintz, IL

Clapp, AR

Melinger, JS

Deschamps, JR

Mattoussi, H

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

[2] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA

[3] USN, Res Lab, Div Elect Sci & Technol, Washington, DC 20375 USA

[4] USN, Res Lab, Struct Matter Lab, Washington, DC 20375 USA

来源：

ADVANCED MATERIALS | 2005年 / 17卷 / 20期

关键词：

D O I：

10.1002/adma.200500722

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

On the surface of a quantum dot (QD), maltose-binding protein (MBP, white and yellow in Figure) labeled with a cyanine dye (red) self-assembles, resulting in Forster resonance energy transfer between the acceptor dye and the OD donor. Upon binding maltose, MBP undergoes a conformational change (white reversible arrow yellow), causing concentration-dependent photoluminescence changes.

引用

页码：2450 / +

页数：7

共 25 条

[11] Manipulation of ligand binding affinity by exploitation of conformational coupling
Marvin, JS
Hellinga, HW
[J]. NATURE STRUCTURAL BIOLOGY, 2001, 8 (09) : 795 - 798
[12] Self-assembly of CdSe-ZnS quantum dot bioconjugates using an engineered recombinant protein
Mattoussi, H
Mauro, JM
Goldman, ER
Anderson, GP
Sundar, VC
Mikulec, FV
Bawendi, MG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (49) : 12142 - 12150
[13] A fluorescence resonance energy transfer-derived structure of a quantum dot-protein bioconjugate nanoassembly
Medintz, IL
Konnert, JH
Clapp, AR
Stanish, I
Twigg, ME
Mattoussi, H
Mauro, JM
Deschamps, JR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (26) : 9612 - 9617
[14] Reversible modulation of quantum dot photoluminescence using a protein-bound photochromic fluorescence resonance energy transfer acceptor
Medintz, IL
Trammell, SA
Mattoussi, H
Mauro, JM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (01) : 30 - 31
[15] Self-assembled nanoscale biosensors based on quantum dot FRET donors
Medintz, IL
Clapp, AR
Mattoussi, H
Goldman, ER
Fisher, B
Mauro, JM
[J]. NATURE MATERIALS, 2003, 2 (09) : 630 - 638
[16] OConnor D., 2012, Time-correlated single-photon counting
[17] Knowledge-based design of reagentless fluorescent biosensors from recombinant antibodies
Renard, M
Belkadi, L
Hugo, N
England, P
Altschuh, D
Bedouelle, H
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2002, 318 (02) : 429 - 442
[18] Detection and characterization of xenon-binding sites in proteins by 129Xe NMR spectroscopy
Rubin, SM
Lee, SY
Ruiz, EJ
Pines, A
Wemmer, DE
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2002, 322 (02) : 425 - 440
[19] Advances in analytical technologies for environmental protection and public safety
Sadik, OA
Wanekaya, AK
Andreescu, S
[J]. JOURNAL OF ENVIRONMENTAL MONITORING, 2004, 6 (06): : 513 - 522
[20] Semiconductor quantum dots for photodynamic therapy
Samia, ACS
Chen, XB
Burda, C
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (51) : 15736 - 15737

← 1 2 3 →