High-yield production of secreted active proteins by the Pseudomonas aeruginosa type III secretion system

被引：15

作者：

Derouazi, M. ^{[1
]}

Toussaint, B. ^{[1
]}

Quenee, L. ^{[1
]}

Epaulard, O. ^{[1
]}

Guillaume, M. ^{[1
]}

Marlu, R. ^{[1
]}

Polack, B. ^{[1
]}

机构：

[1] Univ Grenoble 1, UMR CNRS 5525, Lab TIMC IMAG GREPI TheREx, IFR INSERM 130, Grenoble, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 11期

关键词：

D O I：

10.1128/AEM.02576-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Escherichia coli system is the system of choice for recombinant protein production because it is possible to obtain a high protein yield in inexpensive media. The accumulation of protein in an insoluble form in inclusion bodies remains a major disadvantage. Use of the Pseudomonas aeruginosa type III secretion system can avoid this problem, allowing the production of soluble secreted proteins.

引用

页码：3601 / 3604

页数：4

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