Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom

被引:65
作者
Barros, SF
Friedlanskaia, I
Petricevich, VL
Kipnis, TL
机构
[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biol Reconhecer, BR-28015620 Sao Jose Dos Campos, RJ, Brazil
[2] Inst Butantan, Lab Imunoparasitol, Sao Paulo, Brazil
[3] Inst Butantan, Lab Imunoquim, Sao Paulo, Brazil
关键词
Bothrops atrox; cytokines; animal venoms; mediators of inflammation;
D O I
10.1080/09629359890866
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
WE have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57B1/6 > A/Sn > BALB/c > C3H/HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 mu g) were injected i.p. with 50 mu g of venom produced IL-6, IL-IO, INF-gamma, TNF-alpha and NO in the serum. In vitro the cells from the mice injected and challenged with the venom only released IL-10 while peritoneal macrophages released IL-10, INF-gamma and less amounts of IL-6; (c) establishment of local inflammation and necrosis induced by the venom, coincides with the peaks of TNF-alpha, IFN-gamma and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor. These results suggest that susceptibility to Bothrops atrox venom is genetically dependent but MHC independent; that IL-6, IL-10, TNF-alpha, IFN-gamma and NO can be involved in the mediation of tissue damage; and that the major venom component inducers of the lesions are haemorrhagins.
引用
收藏
页码:339 / 346
页数:8
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