Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom

被引:65
作者
Barros, SF
Friedlanskaia, I
Petricevich, VL
Kipnis, TL
机构
[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Biol Reconhecer, BR-28015620 Sao Jose Dos Campos, RJ, Brazil
[2] Inst Butantan, Lab Imunoparasitol, Sao Paulo, Brazil
[3] Inst Butantan, Lab Imunoquim, Sao Paulo, Brazil
关键词
Bothrops atrox; cytokines; animal venoms; mediators of inflammation;
D O I
10.1080/09629359890866
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
WE have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57B1/6 > A/Sn > BALB/c > C3H/HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 mu g) were injected i.p. with 50 mu g of venom produced IL-6, IL-IO, INF-gamma, TNF-alpha and NO in the serum. In vitro the cells from the mice injected and challenged with the venom only released IL-10 while peritoneal macrophages released IL-10, INF-gamma and less amounts of IL-6; (c) establishment of local inflammation and necrosis induced by the venom, coincides with the peaks of TNF-alpha, IFN-gamma and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor. These results suggest that susceptibility to Bothrops atrox venom is genetically dependent but MHC independent; that IL-6, IL-10, TNF-alpha, IFN-gamma and NO can be involved in the mediation of tissue damage; and that the major venom component inducers of the lesions are haemorrhagins.
引用
收藏
页码:339 / 346
页数:8
相关论文
共 29 条
[21]   Cytokine regulation of endothelial cell function: From molecular level to the bedside [J].
Mantovani, A ;
Bussolino, F ;
Introna, M .
IMMUNOLOGY TODAY, 1997, 18 (05) :231-240
[22]   INTERLEUKIN-10 [J].
MOORE, KW ;
OGARRA, A ;
MALEFYT, RD ;
VIEIRA, P ;
MOSMANN, TR .
ANNUAL REVIEW OF IMMUNOLOGY, 1993, 11 :165-190
[23]  
MULLIGAN MS, 1993, J IMMUNOL, V150, P2401
[24]  
Rosenfeld G., 1971, VENOMOUS ANIMALS THE, V2, P345, DOI [10.1016/b978-0-12-138902-4.50021-0, 10.1016/B978-0-12-138902-4.50021-0]
[25]  
RUFF MR, 1980, J IMMUNOL, V125, P1671
[26]   ENZYMATIC FORMATION OF NITROGEN-OXIDES FROM L-ARGININE IN BOVINE BRAIN CYTOSOL [J].
SCHMIDT, HHHW ;
WILKE, P ;
EVERS, B ;
BOHME, E .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (01) :284-291
[27]  
SCHUMACHER JH, 1988, J IMMUNOL, V141, P1576
[28]   PRO-INFLAMMATORY ACTIVITIES IN ELAPID SNAKE-VENOMS [J].
TAMBOURGI, DV ;
DOSSANTOS, MC ;
FURTADO, MDD ;
DEFREITAS, MCW ;
DASILVA, WD ;
KIPNIS, TL .
BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (03) :723-727
[29]  
*WHO, 1981, WORLD HLTH PROGR CHA