rplC T460C Identified as a Dominant Mutation in Linezolid-Resistant Mycobacterium tuberculosis Strains

被引：80

作者：

Beckert, Patrick

Hillemann, Doris ^{[1
]}

Kohl, Thomas A. ^{[2
]}

Kalinowski, Joern ^{[2
]}

Richter, Elvira ^{[1
]}

Niemann, Stefan

Feuerriegel, Silke ^{[1
]}

机构：

[1] Res Ctr Borstel, Natl Reference Ctr Mycobacteria, Borstel, Germany

[2] Univ Bielefeld, Ctr Biotechnol, Inst Genome Res & Syst Biol, D-33615 Bielefeld, Germany

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 05期

关键词：

OXAZOLIDINONES; MECHANISM;

D O I：

10.1128/AAC.06227-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The ribosomal L3 protein was identified as a novel target in linezolid (LZD)-resistant Mycobacterium tuberculosis strains. Next-generation sequencing confirmed rplC T460C as the sole mutation in an LZD-resistant M. tuberculosis H37Rv strain selected in vitro. Sequencing analysis revealed the rplC T460C mutation in eight further LZD-resistant isolates (three in vitro-selected mutants and five patient isolates, including isolates from three different patients that developed LZD resistance during treatment) but in none of the susceptible control strains (n = 84).

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收藏

页码：2743 / 2745

页数：3

相关论文

共 13 条

[1] Oxazolidinones: activity, mode of action, and mechanism of resistance [J].

Bozdogan, B ;

Appelbaum, PC .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2004, 23 (02) :113-119

[2] In vitro-selected linezolid-resistant Mycobacterium tuberculosis mutants [J].

Hillemann, Doris ;

Ruesch-Gerdes, Sabine ;

Richter, Elvira .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (02) :800-801

[3] Resistance to Linezolid in a Porcine Clostridium perfringens Strain Carrying a Mutation in the rplD Gene Encoding the Ribosomal Protein L4 [J].

Hoelzel, Christina S. ;

Harms, Katrin S. ;

Schwaiger, Karin ;

Bauer, Johann .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2010, 54 (03) :1351-1353

[4] Extensively drug-resistant tuberculosis: current challenges and threats [J].

Jain, Amita ;

Mondal, Rajesh .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2008, 53 (02) :145-150

[5] The site of action of oxazolidinone antibiotics in living bacteria and in human mitochondria [J].

Leach, Karen L. ;

Swaney, Steven M. ;

Colca, Jerry R. ;

McDonald, William G. ;

Blinn, James R. ;

Thomasco, Lisa M. ;

Gadwood, Robert C. ;

Shinabarger, Dean ;

Xiong, Liqun ;

Mankin, Alexander S. .

MOLECULAR CELL, 2007, 26 (03) :393-402

[6] Novel Ribosomal Mutations in Staphylococcus aureus Strains Identified through Selection with the Oxazolidinones Linezolid and Torezolid (TR-700) [J].

Locke, Jeffrey B. ;

Hilgers, Mark ;

Shaw, Karen Joy .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (12) :5265-5274

[7] Mutations in Ribosomal Protein L3 Are Associated with Oxazolidinone Resistance in Staphylococci of Clinical Origin [J].

Locke, Jeffrey B. ;

Hilgers, Mark ;

Shaw, Karen Joy .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (12) :5275-5278

[8] Strategies against multidrug-resistant tuberculosis [J].

Loddenkemper, R ;

Sagebiel, D ;

Brendel, A .

EUROPEAN RESPIRATORY JOURNAL, 2002, 20 :66S-77S

[9] A retrospective TBNET assessment of linezolid safety, tolerability and efficacy in multidrug-resistant tuberculosis [J].

Migliori, G. B. ;

Eker, B. ;

Richardson, M. D. ;

Sotgiu, G. ;

Zellweger, J. -P. ;

Skrahina, A. ;

Ortmann, J. ;

Girardi, E. ;

Hoffmann, H. ;

Besozzi, G. ;

Bevilacqua, N. ;

Kirsten, D. ;

Centis, R. ;

Lange, C. .

EUROPEAN RESPIRATORY JOURNAL, 2009, 34 (02) :387-393

[10]

Perez Federico, 2008, Infect Drug Resist, V1, P27