Activation of T Lymphocytes in Atherosclerotic Plaques

被引:87
作者
Grivel, Jean-Charles [2 ]
Ivanova, Oxana [1 ]
Pinegina, Natalia [1 ]
Blank, Paul S. [3 ]
Shpektor, Alexander [1 ]
Margolis, Leonid B. [2 ]
Vasilieva, Elena [1 ]
机构
[1] Moscow State Univ Med & Dent, Dept Cardiol, Moscow 109240, Russia
[2] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Program Phys Biol, Sect Intercellular Interact, Bethesda, MD USA
[3] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Program Phys Biol, Sect Membranes & Cellular Biophys, Bethesda, MD USA
基金
美国国家卫生研究院;
关键词
atherosclerosis; blood cells; immune system; immunologic techniques; ACUTE CORONARY SYNDROMES; CELLS; INFLAMMATION; EXPRESSION; INFECTIONS; PHENOTYPE; EXPANSION; DISEASE; LESIONS; EVENTS;
D O I
10.1161/ATVBAHA.111.237081
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective-To decipher the immunologic mechanisms of plaque maturation and rupture, it is necessary to analyze the phenotypes and distribution of individual lymphocytes that migrate to the plaques, as well as their activation at different stages of plaque formation. Methods and Results-We developed a protocol to isolate plaque-residing immune cells and analyze their status using polychromatic flow cytometry. We found that the composition and phenotype of T lymphocytes in the plaques differs from that in blood. CD4 and, in particular, CD8(+) T cells in plaques are highly activated; the fraction of CD8 T cells coexpressing CD25 and human leukocyte antigen-D related in plaques was 6 times as large as in blood. Conclusion-The first flow-cytoanalysis of individual T cells in atherosclerotic plaques indicates that plaques represent a separate immunologic compartment from blood with lymphocytes characterized by a high level of T-cell activation, which is compatible with the presence of antigen(s) that trigger infiltration activation of these cells. The ability to isolate and characterize these cells may lead to the identification of such antigens. (Arterioscler Thromb Vasc Biol. 2011;31:2929-2937.)
引用
收藏
页码:2929 / 2937
页数:9
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