Comparison of a pneumococcal common protein (PsaA) antibody ELISA and a PsaA immune complex ELISA for detection of pneumococcal serum antibody

被引：16

作者：

Tharpe, JA

Russell, H

Leinonen, M

Plikaytis, BD

Breiman, RF

Carlone, GM

Ades, EW

Sampson, JS

机构：

[1] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Bacterial & Mycot Dis, Resp Dis Branch, Atlanta, GA 30333 USA

[2] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Bacterial & Mycot Dis, Biostat & Informat Management Branch, Atlanta, GA 30333 USA

[3] Natl Publ Hlth Inst, Helsinki, Finland

来源：

PATHOBIOLOGY | 1998年 / 66卷 / 02期

关键词：

pneumococcal surface adhesin A; pneumococcal pneumonia; ELISA; immune complexes;

D O I：

10.1159/000028000

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We examined and compared results from three assays, an enzyme-linked immunosorbent assay (ELISA) and two immune complex ELISAs for analysis of the serum antibody response to a native pneumococcal 37-kD common cell-wall protein by using acute-and convalescent-phase sera from 56 patients with community-acquired pneumonia. The sensitivities of the ELISA, the undissociated and dissociated immune complex assays were 85% (23 of 27), 78% (21 of 27) and 67% (18 of 27), respectively. To determine specificity, paired sera from patients with pneumonia of other bacterial etiologies were tested. The specificities were 83, 83 and 72% for the ELISA, undissociated immune complex, and dissociated immune complex, respectively. Based on this study, the sensitivities of the three assays were not statistically different. These tests could be used retrospectively to confirm invasive pneumococcal disease.

引用

页码：77 / 83

页数：11

共 30 条

[1] ETIOLOGY OF COMMUNITY-ACQUIRED PNEUMONIA IN PATIENTS REQUIRING HOSPITALIZATION [J].

BERNTSSON, E ;

BLOMBERG, J ;

LAGERGARD, T ;

TROLLFORS, B .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1985, 4 (03) :268-272

[2] PNEUMOCOCCAL BACTEREMIA IN CHARLESTON COUNTY, SOUTH-CAROLINA - A DECADE LATER [J].

BREIMAN, RF ;

SPIKA, JS ;

NAVARRO, VJ ;

DARDEN, PM ;

DARBY, CP .

ARCHIVES OF INTERNAL MEDICINE, 1990, 150 (07) :1401-1405

[3] DIAGNOSIS OF PNEUMONIA BY CULTURES, BACTERIAL AND VIRAL-ANTIGEN DETECTION TESTS, AND SEROLOGY WITH SPECIAL REFERENCE TO ANTIBODIES AGAINST PNEUMOCOCCAL ANTIGENS [J].

BURMAN, LA ;

TROLLFORS, B ;

ANDERSSON, B ;

HENRICHSEN, J ;

JUTO, P ;

KALLINGS, I ;

LAGERGARD, T ;

MOLLBY, R ;

NORRBY, R .

JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (05) :1087-1093

[4]

*CDCP, 1997, MORBID MORTAL WKLY R, V46

[5] DEMONSTRATION OF CIRCULATING PNEUMOCOCCAL IMMUNOGLOBULIN-G IMMUNE-COMPLEXES IN PATIENTS WITH COMMUNITY-ACQUIRED PNEUMONIA BY MEANS OF AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].

HOLLOWAY, Y ;

SNIJDER, JAM ;

BOERSMA, WG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (12) :3247-3254

[6] IDENTIFICATION OF A PATIENT WITH STREPTOCOCCUS-PNEUMONIAE BACTEREMIA AND MENINGITIS BY THE POLYMERASE CHAIN-REACTION (PCR) [J].

ISAACMAN, DJ ;

ZHANG, YZ ;

RYDQUISTWHITE, J ;

WADOWSKY, RM ;

POST, JC ;

EHRLICH, GD .

MOLECULAR AND CELLULAR PROBES, 1995, 9 (03) :157-160

[7] ACUTE NEPHRITIS AND PULMONARY ALVEOLITIS FOLLOWING PNEUMOCOCCAL PNEUMONIA [J].

KAEHNY, WD ;

OZAWA, T ;

SCHWARZ, MI ;

STANFORD, RE ;

KOHLER, PF ;

MCINTOSH, RM .

ARCHIVES OF INTERNAL MEDICINE, 1978, 138 (05) :806-808

[8]

KAIJSER B, 1979, J INFECT S2, V1, P39

[9] DIAGNOSIS OF PNEUMOCOCCAL PNEUMONIA BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY OF ANTIBODIES TO PNEUMOCOCCAL HEMOLYSIN (PNEUMOLYSIN) [J].

KALIN, M ;

KANCLERSKI, K ;

GRANSTROM, M ;

MOLLBY, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (02) :226-229

[10] DIAGNOSIS OF PNEUMOCOCCAL PNEUMONIA - A COMPARISON BETWEEN MICROSCOPIC EXAMINATION OF EXPECTORATE, ANTIGEN-DETECTION AND CULTURAL PROCEDURES [J].

KALIN, M ;

LINDBERG, AA .

SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 1983, 15 (03) :247-255

← 1 2 3 →