Purification by centrifugal partition chromatography of amphiphilic compounds, glycolipids and pseudo-glycolipids synthesized by using cells

被引：8

作者：

Kato, Tomohisa ^{[2
,3
]}

Miyagawa, Atsushi ^{[1
,2
]}

Kasuya, Maria Carmelita Z. ^{[1
]}

Ito, Ayaka ^{[1
]}

Hatanaka, Kenichi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

[2] Japan Chem Innovat Inst, Chiyoda Ku, Tokyo 1010051, Japan

[3] Kaneka Corp, Kita Ku, Osaka 5308288, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1178卷 / 1-2期

关键词：

glycolipids; neutral lipid; acidic lipid; ganglioside; centrifugal partition chromatograph;

D O I：

10.1016/j.chroma.2007.11.094

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Centrifugal partition chromatography (CPC) was applied to separate amphiphilic glycolipids and pseudo-glycolipids synthesized by using cells. Neutral and acidic lipid fractions were isolated by CPC under suitable conditions respectively. Separation of neutral lipid, Gb3-type and Gb4-type oligosaccharide synthesized by using cells, was performed with a two-phase solvent system composed of chloroform-methanol-water at a volume ratio of 5:6:4. On the other hand, separation of acidic lipid, GM3-type oligosaccharide synthesized by using cells, and ganglioside extracted from rat brain were performed with a two-phase solvent system composed of butanol - ethanol - 1% acetic acid at a volume ratio of 4:1:5. 8.3 mg of Gb3 analogue, 5.1 mg of Gb4 analogue, and 19.5 mg of GM3 analogue were purified from 3.21 of culture medium obtained by incubation of African green-monkey kidney (Vero) cells with 50 mu M n-dodecyl beta-lactoside using CPC. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：154 / 159

页数：6

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