Syntheses of oligosaccharides using cell function

被引：22

作者：

Sato, Toshinori ^{[1
]}

Hatanaka, Kenichi

Hashimoto, Hironobu

Yamagata, Tatsuya

机构：

[1] Keio Univ, Dept Biosci & Informat, Kouhoku Ku, Yokohama, Kanagawa 2238522, Japan

[2] Univ Tokyo, CCR, Tokyo 1538505, Japan

[3] Univ Tokyo, IIS, Tokyo 1538505, Japan

[4] Shenyang Pharmaceut Univ, Lab Tumor Biol & Glycobiol, Shenyang 110016, Peoples R China

来源：

TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY | 2007年 / 19卷 / 105期

关键词：

saccharide primer; glycan synthesis; biocombinatorial synthesis; oligosaccharide; glycan array;

D O I：

10.4052/tigg.19.1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The authors developed a method of synthesis of oligosaccharides using cell function. By administering the saccharide primer that is amphiphilic alkylglycoside into cell culture medium, glycosylated products were collected from the medium after 1-2 days. The sequences of the products were dependent on the biosynthetic pathway of sugars in the cells. By combining the saccharide primer and cell lines, it was possible to synthesize many kinds of oligosaccharides. The introduction of a functional group in an alkyl chain of saccharide primer made it possible to polymerize and immobilize to solid support. The saccharide primer method is expected to become a new methodology for glycomics.

引用

页码：1 / 17

页数：17

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