Determination of myristicin in rat serum samples using microbore high performance liquid chromatography with column-switching

被引：13

作者：

Lee, HS ^{[1
]}

Kim, JH

Kim, K

Do, KS

机构：

[1] Wonkwang Univ, Coll Pharm, Iksan 570749, South Korea

[2] Korea Res Inst Chem Technol, Toxicol Res Ctr, Taejon 305606, South Korea

[3] Taejon Hlth & Environm Res Inst, Taejon 305153, South Korea

来源：

CHROMATOGRAPHIA | 1998年 / 48卷 / 5-6期

关键词：

column liquid chromatography; microbore columns; column-switching; myristicin; serum;

D O I：

10.1007/BF02467705

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microbore high-performance liquid chromatographic method with column-switching was developed for the analysis of myristicin from rat serum without prepurification. Deproteinization, fractionation, concentration and separation of analyte were carried out by appropriate switching of columns and using solvent mixtures. The method showed excellent precision, accuracy and speed with a detection limit of 10 ng mL(-1) from 25 mu L of serum. The total analysis time per sample was 25 min and the coefficients of variation for intra- and inter-assay were less than 1.8 %.

引用

页码：365 / 368

页数：4

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