Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum

被引：65

作者：

Vasicová, P

Abrhámová, Z

Nesvera, J

Pátek, M

Sahm, H

Eikmanns, B

机构：

[1] Acad Sci Czech Republ, Inst Microbiol, CZ-14220 Prague 4, Czech Republic

[2] Forschungszentrum Julich, Inst Biotechnol, D-52425 Julich, Germany

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 10期

关键词：

Corynebacterium glutamicum; promoters; integrative vector;

D O I：

10.1023/A:1008827609914

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Autonomously replicating shuttle (Escherichia coli - Corynebacterium glutamicum) promoter-probe vector pET2 and integrative promoter-probe vector pRIM2 for C. glutamicum were constructed. Transcriptional fusions of promoter-carrying fragments to the promoterless chloramphenicol acetyltransferase gene (cat) carried by the vectors can be used to determine position, strength and regulation of the respective promoters in multicopy system (pET2) and in single-copy system (pRIM2) and to perform deletion and mutation studies of the promoters. Utility of the vectors was shown on three C, glutamicum promoters.

引用

页码：743 / 746

页数：4

共 17 条

[1] CONSTRUCTION OF A PROMOTER-PROBE SHUTTLE VECTOR FOR ESCHERICHIA-COLI AND BREVIBACTERIA [J].