Preparation and properties of vesicles formed from phospholipid analogues of N-(phosphonoacetyl)-L-aspartate (PALA) by sonication or extrusion:: Transition temperature, particle size, glucose entrapment, and 31P NMR

被引:8
作者
Oliger, P
Hebrant, M
Grison, C
Coutrot, P
Tondre, C
机构
[1] Univ Nancy 1, Inst Nanceien Chim Mol, Lab Chim Phys Organ & Colloidale, UMR CNRS 7565, F-54506 Vandoeuvre Les Nancy, France
[2] Univ Nancy 1, Inst Nanceien Chim Mol, Lab Chim Organ 2, UMR CNRS 7565, F-54506 Vandoeuvre Les Nancy, France
关键词
D O I
10.1021/la010332x
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We characterize here the ability of new amphiphilic compounds to form vesicles that could potentially be used as vectors for therapeutical applications. These compounds are derived from N-phosphonoacetyl-L-aspartate (PALA), a potential antitumoral agent. Vesicular dispersions of phospholipid analogues of PALA with different alkyl chain lengths (diC(12)-, diC(14)-, diC(16)-, and diC(18)-PALA), which had been previously synthesized, are tested from different points of view. Two kinds of preparation methods are compared: sonication; extrusion. The two preparation methods resulted in important differences in the properties of the dispersions which confirmed the conclusions of a previous investigation from cryo-TEM imaging. The results of fluorescence polarization experiments, performed either with the pure compounds or their mixtures with lecithins or cholesterol, indicated that the transition temperature T. can be modulated if it is required for the applications. The sonicated particles showed smaller size and much lower glucose encapsulation than the extruded particles. In both cases these properties are only weakly depending on the amphiphile alkyl chain lengths. The glucose permeability has appeared to considerably increase (lower encapsulation) at T > T-m. The effect of Mn2+ on the P-31 NMR signal intensity suggested that unilamellar vesicles are coexisting with other types of particle in case of extrusion, whereas in case of sonication only few and less stable vesicles are present, in agreement with the preceding observations.
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页码:6426 / 6432
页数:7
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