Application of analytical and preparative high-speed counter-current chromatography for separation of alkaloids from Coptis chinensis Franch

被引：91

作者：

Yang, FQ

Zhang, TY

Zhang, R

Ito, Y

机构：

[1] NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA

[2] Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China

[3] Beijing Union Univ, Chem Engn Coll, Beijing 100029, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 829卷 / 1-2期

关键词：

Coptis chinensis Franch; high-speed counter-current chromatography; alkaloids;

D O I：

10.1016/S0021-9673(98)00776-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Analytical high-speed counter-current chromatography (HSCCC) was used for the systematic selection and optimization of the two-phase solvent system to separate alkaloids from Coptis chinensis Franch. The optimum solvent system thus obtained led to the successful separation of alkaloids from C. chinensis Franch by preparative HSCCC. One batch separation yielded four pure alkaloids, including palmatine, berberine, epiberberine and coptisine from the crude alkaloid extract. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：137 / 141

页数：5

共 18 条

[1] ANALYTICAL HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY WITH A COIL PLANET CENTRIFUGE [J].