Synthesis of an antisense oligonucleotide targeted against C-raf kinase: Efficient oligonucleotide synthesis without chlorinated solvents

被引：29

作者：

Krotz, AH ^{[1
]}

Cole, DL ^{[1
]}

Ravikumar, VT ^{[1
]}

机构：

[1] ISIS Pharmaceut, Carlsbad, CA 92008 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 1999年 / 7卷 / 03期

关键词：

D O I：

10.1016/S0968-0896(98)00253-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is demonstrated that a solution of dichloroacetic acid in toluene removes dimethoxytrityl groups from the 5'-terminus of an antisense phosphorothioate oligodeoxyribonucleotide (ISIS 5132/CGP69846A) during synthesis on solid support cleanly and efficiently. It is therefore suggested to replace health hazardous dichloromethane which is typically used in oligonucleotide synthesis as solvent for DMTr-removal by toluene. (C) 1999 Elsevier Science Ltd. All rights reserved.

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页码：435 / 439

页数：5

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