Phosphorothioate oligonucleotides: Largely reduced (n-1)-mer and phosphodiester content through the use of dimeric phosphoramidite synthons

被引：27

作者：

Krotz, AH ^{[1
]}

Klopchin, P ^{[1
]}

Cole, DL ^{[1
]}

Ravikumar, VT ^{[1
]}

机构：

[1] ISIS PHARMACEUT,CARLSBAD,CA 92008

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1997年 / 7卷 / 01期

关键词：

D O I：

10.1016/S0960-894X(96)00573-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Phosphorothioate oligonucleotides synthesized through an assembly of dimeric phosphoramidite synthons on controlled pore glass solid support show a significantly improved impurity profile compared to oligomers synthesized through a coupling of standard monomer phosphoramidites. A greater than 70% reduction of the (n-1)-mer population and a ca 50% reduction of phosphodiester linkages has been achieved. Copyright (C) 1996 Elsevier Science Ltd

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页码：73 / 78

页数：6

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