IMPROVED PROCEDURE FOR THE REDUCTION OF N-1 CONTENT IN SYNTHETIC OLIGONUCLEOTIDES

被引：12

作者：

IYER, RP

YU, D

JIANG, ZW

AGRAWAL, S

机构：

[1] Hybridon Inc., One Innovation Drive, Worcester

来源：

NUCLEOSIDES & NUCLEOTIDES | 1995年 / 14卷 / 06期

关键词：

D O I：

10.1080/15257779508010696

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By incorporating a ''capping step'' at the start of an oligonucleotide synthesis (''pre-cap'') and following a ''SUP'' work-up protocol with ammonium hydroxide, an overall improvement is observed in the quality of oligonucleotides synthesized on a large scale on controlled pore glass support (CPG). Rationalization of these results is provided.

引用

收藏

页码：1349 / 1357

页数：9

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