Development and Validation of a Rapid Immunochromatographic Assay for Detection of Middle East Respiratory Syndrome Coronavirus Antigen in Dromedary Camels

被引：36

作者：

Song, Daesub ^{[1
]}

Ha, Gunwoo ^{[2
]}

Serhan, Wissam ^{[3
]}

Eltahir, Yassir ^{[4
]}

Yusof, Mohammed ^{[3
]}

Hashem, Farouq ^{[3
]}

Elsayed, Elsaeid ^{[5
]}

Marzoug, Bahaaeldin ^{[5
]}

Abdelazim, Assem ^{[5
]}

Al Muhairi, Salama ^{[3
]}

机构：

[1] Univ Sci & Technol, Korea Res Inst Biosci & Biotechnol, Viral Infect Dis Res Ctr, Taejon, South Korea

[2] BioNote Inc, Hwaseong Si, Gyeonggi Do, South Korea

[3] Abu Dhabi Food Control Author, Anim Wealth Sect, Vet Labs Div, Abu Dhabi, U Arab Emirates

[4] Abu Dhabi Food Control Author, Anim Wealth Sect, Epidemiol Sect, Abu Dhabi, U Arab Emirates

[5] Abu Dhabi Food Control Author, Anim Wealth Sect, Vet Serv Sect, Abu Dhabi, U Arab Emirates

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 04期

关键词：

D O I：

10.1128/JCM.03096-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.

引用

页码：1178 / 1182

页数：5

共 17 条

[1]

Abd El Wahed A, 2013, PLOS ONE, V8, DOI [10.1371/journal.pone.0071642, 10.1371/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364]

[2] Human Betacoronavirus 2c EMC/2012-related Viruses in Bats, Ghana and Europe [J].

Annan, Augustina ;

Baldwin, Heather J. ;

Corman, Victor Max ;

Klose, Stefan M. ;

Owusu, Michael ;

Nkrumah, Evans Ewald ;

Badu, Ebenezer Kofi ;

Anti, Priscilla ;

Agbenyega, Olivia ;

Meyer, Benjamin ;

Oppong, Samuel ;

Sarkodie, Yaw Adu ;

Kalko, Elisabeth K. V. ;

Lina, Peter H. C. ;

Godlevska, Elena V. ;

Reusken, Chantal ;

Seebens, Antje ;

Gloza-Rausch, Florian ;

Vallo, Peter ;

Tschapka, Marco ;

Drosten, Christian ;

Drexler, Jan Felix .

EMERGING INFECTIOUS DISEASES, 2013, 19 (03) :456-459

[3]

[Anonymous], EURO SURVEILL

[4]

[Anonymous], 2013, EURO SURVEILL

[5]

[Anonymous], 2014, MIDDL E RESP SYNDR C

[6]

[Anonymous], 1988, Antibodies: A laboratory manual

[7] Evidence for Camel-to-Human Transmission of MERS Coronavirus [J].

Azhar, Esam I. ;

El-Kafrawy, Sherif A. ;

Farraj, Suha A. ;

Hassan, Ahmed M. ;

Al-Saeed, Muneera S. ;

Hashem, Anwar M. ;

Madani, Tariq A. .

NEW ENGLAND JOURNAL OF MEDICINE, 2014, 370 (26) :2499-2505

[8]

Corman VM, 2012, EURO SURVEILL, V17

[9] Validation of serological assays for diagnosis of infectious diseases [J].

Jacobson, RH .

REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES, 1998, 17 (02) :469-486

[10] Development and preliminary application of an immunochromatographic strip for rapid detection of infection with porcine reproductive and respiratory syndrome virus in swine [J].

Li, Xue-song ;

Fu, Fang ;

Lang, Yue-kun ;

Li, Hai-zhong ;

Wang, Wei ;

Chen, Xin ;

Tian, Hua-bin ;

Zhou, Yan-jun ;

Tong, Guang-zhi ;

Li, Xi .

JOURNAL OF VIROLOGICAL METHODS, 2011, 176 (1-2) :46-52

← 1 2 →