Progressive ordering with decreasing temperature of the phospholipids of influenza virus

被引:183
作者
Polozov, Ivan V. [1 ]
Bezrukov, Ludmila [1 ]
Gawrisch, Klaus [2 ]
Zimmerberg, Joshua [1 ]
机构
[1] NICHHD, Lab Cellular & Mol Biophys, Bethesda, MD 20892 USA
[2] NIAAA, NIH, Lab Membrane Biochem & Biophys, Bethesda, MD 20892 USA
关键词
D O I
10.1038/nchembio.77
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using linewidth and spinning sideband intensities of lipid hydrocarbon chain resonances in proton magic angle spinning NMR spectra, we detected the temperature-dependent phase state of naturally occurring lipids of intact influenza virus without exogenous probes. Increasingly, below 41 degrees C ordered and disordered lipid domains coexisted for the viral envelope and extracts thereof. At 22 degrees C much lipid was in a gel phase, the fraction of which reversibly increased with cholesterol depletion. Diffusion measurements and fluorescence microscopy independently confirmed the existence of gel-phase domains. Thus the existence of ordered regions of lipids in biological membranes is now demonstrated. Above the physiological temperatures of influenza infection, the physical properties of viral envelope lipids, regardless of protein content, were indistinguishable from those of the disordered fraction. Viral fusion appears to be uncorrelated to ordered lipid content. Lipid ordering may contribute to viral stability at lower temperatures, which has recently been found to be critical for airborne transmission.
引用
收藏
页码:248 / 255
页数:8
相关论文
共 50 条
[1]   Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension [J].
Baumgart, T ;
Hess, ST ;
Webb, WW .
NATURE, 2003, 425 (6960) :821-824
[2]   Mass spectrometric analysis reveals an increase in plasma membrane polyunsaturated phospholipid species upon cellular cholesterol loading [J].
Blom, TS ;
Koivusalo, M ;
Kuismanen, E ;
Kostiainen, R ;
Somerharju, P ;
Ikonen, E .
BIOCHEMISTRY, 2001, 40 (48) :14635-14644
[3]   Structure and origin of ordered lipid domains in biological membranes [J].
Brown, DA ;
London, E .
JOURNAL OF MEMBRANE BIOLOGY, 1998, 164 (02) :103-114
[4]   THE MOLECULAR-ORGANIZATION OF THE INFLUENZA-VIRUS SURFACE - STUDIES USING PHOTOREACTIVE AND FLUORESCENT LABELED PHOSPHOLIPID PROBES [J].
BUKRINSKAYA, AG ;
MOLOTKOVSKY, JG ;
VODOVOZOVA, EL ;
MANEVICH, YM ;
BERGELSON, LD .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 897 (02) :285-292
[5]   The raft-promoting property of virion-associated cholesterol, but not the presence of virion-associated Brij 98 rafts, is a determinant of human immunodeficiency virus type 1 infectivity [J].
Campbell, S ;
Gaus, K ;
Bittman, R ;
Jessup, W ;
Crowe, S ;
Mak, J .
JOURNAL OF VIROLOGY, 2004, 78 (19) :10556-10565
[6]   The diversity of the liquid ordered (Lo) phase of phosphatidylcholine/cholesterol membranes:: A variable temperature multinuclear solid-state NMR and X-ray diffraction study [J].
Clarke, JA ;
Heron, AJ ;
Seddon, JM ;
Law, RV .
BIOPHYSICAL JOURNAL, 2006, 90 (07) :2383-2393
[7]   Lipid rafts reconstituted in model membranes [J].
Dietrich, C ;
Bagatolli, LA ;
Volovyk, ZN ;
Thompson, NL ;
Levi, M ;
Jacobson, K ;
Gratton, E .
BIOPHYSICAL JOURNAL, 2001, 80 (03) :1417-1428
[8]   Sphingomyelin structure influences the lateral diffusion and raft formation in lipid bilayers [J].
Filippov, A ;
Orädd, G ;
Lindblom, G .
BIOPHYSICAL JOURNAL, 2006, 90 (06) :2086-2092
[9]   The effect of cholesterol on the lateral diffusion of phospholipids in oriented bilayers [J].
Filippov, A ;
Orädd, G ;
Lindblom, G .
BIOPHYSICAL JOURNAL, 2003, 84 (05) :3079-3086
[10]  
FOLCH J, 1957, J BIOL CHEM, V226, P497