Photodestruction intermediates probed by an adjacent reporter molecule

被引：26

作者：

Ha, T ^{[1
]}

Xu, J ^{[1
]}

机构：

[1] Univ Illinois, Dept Phys, Urbana, IL 61801 USA

来源：

PHYSICAL REVIEW LETTERS | 2003年 / 90卷 / 22期

关键词：

D O I：

10.1103/PhysRevLett.90.223002

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We used a fluorescence resonance energy transfer donor molecule to probe the multiple intermediates in the photoinduced destruction of an acceptor molecule. These intermediates are nonemitting but are still able to quench the fluorescence of the donor at a distance scale shorter than conventional fluorescence resonance energy transfer, suggesting novel biophysical applications.

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页数：4

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共 23 条

[1] ALTERATIONS OF SINGLE-MOLECULE FLUORESCENCE LIFETIMES IN NEAR-FIELD OPTICAL MICROSCOPY [J].

AMBROSE, WP ;

GOODWIN, PM ;

MARTIN, JC ;

KELLER, RA .

SCIENCE, 1994, 265 (5170) :364-367

[2]

Bernacchi S, 2001, Nucleic Acids Res, V29, pE62, DOI 10.1093/nar/29.13.e62

[3] E-coli Rep oligomers are required to initiate DNA unwinding in vitro [J].

Cheng, W ;

Hsieh, J ;

Brendza, KM ;

Lohman, TM .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (02) :327-350

[4] Investigation of molecular dimers by ensemble and single molecule spectroscopy [J].

Christ, T ;

Petzke, F ;

Bordat, P ;

Herrmann, A ;

Reuther, E ;

Müllen, K ;

Basché, T .

JOURNAL OF LUMINESCENCE, 2002, 98 (1-4) :23-33

[5] Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2 [J].

Deniz, AA ;

Laurence, TA ;

Beligere, GS ;

Dahan, M ;

Martin, AB ;

Chemla, DS ;

Dawson, PE ;

Schultz, PG ;

Weiss, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (10) :5179-5184

[6] Single-pair fluorescence resonance energy transfer on freely diffusing molecules: Observation of Forster distance dependence and subpopulations [J].

Deniz, AA ;

Dahan, M ;

Grunwell, JR ;

Ha, TJ ;

Faulhaber, AE ;

Chemla, DS ;

Weiss, S ;

Schultz, PG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) :3670-3675

[7] Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor [J].

Ha, T ;

Enderle, T ;

Ogletree, DF ;

Chemla, DS ;

Selvin, PR ;

Weiss, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6264-6268

[8] Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase [J].

Ha, T ;

Rasnik, I ;

Cheng, W ;

Babcock, HP ;

Gauss, GH ;

Lohman, TM ;

Chu, S .

NATURE, 2002, 419 (6907) :638-641

[9] Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism [J].

Ha, TJ ;

Ting, AY ;

Liang, J ;

Caldwell, WB ;

Deniz, AA ;

Chemla, DS ;

Schultz, PG ;

Weiss, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (03) :893-898

[10] Mg2+-dependent conformational change of RNA studied by fluorescence correlation and FRET on immobilized single molecules [J].

Kim, HD ;

Nienhaus, GU ;

Ha, T ;

Orr, JW ;

Williamson, JR ;

Chu, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (07) :4284-4289