Quantitative imaging microscopy for the sensitive detection of administered metal containing drugs in single cells and tissue slices - a demonstration using platinum based chemotherapeutic agents

被引：12

作者：

Mauthe, RJ

Sideras-Haddad, E

Turteltaub, KW

Bench, G ^{[1
]}

机构：

[1] Univ Calif Lawrence Livermore Natl Lab, Biol & Biotechnol Res Program, Livermore, CA 94550 USA

[2] Univ Calif Lawrence Livermore Natl Lab, Ctr Accelerator Mass Spectrometry, Livermore, CA 94550 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1998年 / 17卷 / 4-5期

关键词：

cisplatin; carboplatin; nuclear microscopy; particle induced X-ray emission (PIXE); single cell analysis; quantitative metal detection;

D O I：

10.1016/S0731-7085(97)00225-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe the use of Nuclear microscopy (microbeam PIXE) for the quantitative micron scale analysis of platinum based chemotherapeutic agents in individual cells and tissue slices. We demonstrate that microbeam PIXE has the sensitivity and accuracy to quantitatively measure the uptake of the chemotherapeutic agent cis-diamminedichloroplatinum(II) (cisplatin) and monitor other endogenous metal contents in single cells in a time- and dose-dependent fashion. Additionally, the technique can quantitatively image therapeutic levels of cisplatin and cisplatin analogs including cis-diammine[1,1-cyclobutanedicarboxylato]platinum(II) (carboplatin) in tissues from an animal model. This quantitative imaging microscopy has general application for the sensitive measurement of metal containing drugs/compounds at the cellular level and allows the study of cellular distribution and mechanism of action related to toxic response and cell function. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：651 / 663

页数：13

共 31 条

[11] CHARACTERIZATION OF A DNA DAMAGE-RECOGNITION PROTEIN FROM MAMMALIAN-CELLS THAT BINDS SPECIFICALLY TO INTRASTRAND D(GPG) AND D(APG) DNA ADDUCTS OF THE ANTICANCER DRUG CISPLATIN [J].