Nanoparticle-based electrochemical detection of hepatitis B virus using stripping chronopotentiometry

被引：30

作者：

Hanaee, H. ^{[1
]}

Ghourchian, H. ^{[1
]}

Ziaee, A-A. ^{[1
]}

机构：

[1] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran

来源：

ANALYTICAL BIOCHEMISTRY | 2007年 / 370卷 / 02期

关键词：

gold nanoparticles; paramagnetic beads; silver enhancement; stripping chronopotentiometry; hepatitis B virus;

D O I：

10.1016/j.ab.2007.06.029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A selective and sensitive gold nanoparticle-based electrochemical method for detection of hepatitis B virus DNA sequences was used. This method relies on the hybridization of amplified hepatitis B virus DNA strands with probes that are extended on paramagnetic beads. After separation of noncomplementary sequences, hybridized magnetic beads were treated with streptavidin-modified gold followed by silver enhancement. High selectivity and high sensitivity were obtained using electrochemical stripping detection of silver ions that were deposited on gold nanoparticles. With a signal/noise ratio of approximately 4.6, the detection limit was estimated to be 0.7 ng/ml. (c) 2007 Elsevier Inc. All rights reserved.

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收藏

页码：195 / 200

页数：6

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