Porphyromonas gingivalis is a major pathogen in destructive periodontal disease in humans. Detection and quantification of this microorganism are relevant for diagnosis and treatment planning. The prevalence and quantity of P. gingivalis in subgingival plaque samples of periodontitis patients were determined by anaerobic culture and real-time PCR amplification of the 16S small-subunit rRNA gene. The PCR was performed with primers and a fluorescently labeled probe specific for the P. gingivalis 16S rRNA gene. By the real-time PCR assay, as few as 1 CFU of P. gingivalis could be detected. Subgingival plaque samples from 259 adult patients with severe periodontitis were analyzed. P. gingivalis was detected in 111 (43%) of the 259 subgingival plaque samples by culture and in 138 (53%) samples by PCR. The sensitivity, specificity, and positive and negative predictive values of the real-time PCR were 100, 94, 94, and 100%, respectively. We conclude that real-time PCR confirms the results of quantitative culture of P. gingivalis and offers significant advantages with respect to the rapidity and sensitivity of detection of P. gingivalis in subgingival plaque samples.
引用
收藏
页码:4950 / 4954
页数:5
相关论文
共 38 条
[31]
van Foreest A, 2002, TIJDSCHR DIERGENEESK, V127, P554
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
VANWINKELHOFF, AJ
VANSTEENBERGEN, TJM
论文数: 0引用数: 0
h-index: 0
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
VANSTEENBERGEN, TJM
KIPPUW, N
论文数: 0引用数: 0
h-index: 0
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
KIPPUW, N
DEGRAAFF, J
论文数: 0引用数: 0
h-index: 0
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
VANWINKELHOFF, AJ
VANSTEENBERGEN, TJM
论文数: 0引用数: 0
h-index: 0
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
VANSTEENBERGEN, TJM
KIPPUW, N
论文数: 0引用数: 0
h-index: 0
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS
KIPPUW, N
DEGRAAFF, J
论文数: 0引用数: 0
h-index: 0
机构:
FREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDSFREE UNIV AMSTERDAM,SCH MED,DEPT MED MICROBIOL,COMMENSAL INFECT RES GRP,1007 MC AMSTERDAM,NETHERLANDS