Comparison of real-time PCR and culture for detection of Porphyromonas gingivalis in subgingival plaque samples

被引:127
作者
Boutaga, K
van Winkelhoff, AJ
Vandenbroucke-Grauls, CMJE
Savelkoul, PHM
机构
[1] Free Univ Amsterdam Hosp, Ctr Med, Dept Med Microbiol & Infect Control, NL-1007 MB Amsterdam, Netherlands
[2] Acad Ctr Dent Amsterdam, Sect Oral Microbiol, Dept Pedodont, NL-1066 EA Amsterdam, Netherlands
关键词
D O I
10.1128/JCM.41.11.4950-4954.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Porphyromonas gingivalis is a major pathogen in destructive periodontal disease in humans. Detection and quantification of this microorganism are relevant for diagnosis and treatment planning. The prevalence and quantity of P. gingivalis in subgingival plaque samples of periodontitis patients were determined by anaerobic culture and real-time PCR amplification of the 16S small-subunit rRNA gene. The PCR was performed with primers and a fluorescently labeled probe specific for the P. gingivalis 16S rRNA gene. By the real-time PCR assay, as few as 1 CFU of P. gingivalis could be detected. Subgingival plaque samples from 259 adult patients with severe periodontitis were analyzed. P. gingivalis was detected in 111 (43%) of the 259 subgingival plaque samples by culture and in 138 (53%) samples by PCR. The sensitivity, specificity, and positive and negative predictive values of the real-time PCR were 100, 94, 94, and 100%, respectively. We conclude that real-time PCR confirms the results of quantitative culture of P. gingivalis and offers significant advantages with respect to the rapidity and sensitivity of detection of P. gingivalis in subgingival plaque samples.
引用
收藏
页码:4950 / 4954
页数:5
相关论文
共 38 条
  • [31] van Foreest A, 2002, TIJDSCHR DIERGENEESK, V127, P554
  • [32] Porphyromonas gingivalis, Bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction
    van Winkelhoff, AJ
    Loos, BG
    van der Reijden, WA
    van der Velden, U
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 2002, 29 (11) : 1023 - 1028
  • [33] Discrepancy between culture and DNA probe analysis for the detection of periodontal bacteria
    vanSteenbergen, TJM
    Timmerman, MF
    Mikx, FHM
    deQuincey, G
    vanderWeijden, GA
    vanderVelden, U
    deGraaff, J
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 1996, 23 (10) : 955 - 959
  • [34] RAPID CHARACTERIZATION OF ORAL AND NONORAL PIGMENTED BACTEROIDES SPECIES WITH THE ATB ANAEROBES ID SYSTEM
    VANWINKELHOFF, AJ
    CLEMENT, M
    DEGRAAFF, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (05) : 1063 - 1065
  • [35] FURTHER CHARACTERIZATION OF BACTEROIDES-ENDODONTALIS, AN ASACCHAROLYTIC BLACK-PIGMENTED BACTEROIDES SPECIES FROM THE ORAL S-CAVITY
    VANWINKELHOFF, AJ
    VANSTEENBERGEN, TJM
    KIPPUW, N
    DEGRAAFF, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (01) : 75 - 79
  • [36] VANWINKELHOFF AJ, 1988, J CLIN PERIODONTOL, V15, P145
  • [37] NATURAL DISTRIBUTION OF 5 BACTERIA ASSOCIATED WITH PERIODONTAL-DISEASE
    WOLFF, LF
    AEPPLI, DM
    PIHLSTROM, B
    ANDERSON, L
    STOLTENBERG, J
    OSBORN, J
    HARDIE, N
    SHELBURNE, C
    FISCHER, G
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 1993, 20 (10) : 699 - 706
  • [38] THE LABORATORY DIAGNOSIS OF PERIODONTAL INFECTIONS
    ZAMBON, JJ
    HARASZTHY, VI
    [J]. PERIODONTOLOGY 2000, 1995, 7 : 69 - 82