Gas-phase basicities of deprotonated matrix-assisted laser desorption/ionization matrix molecules

被引：62

作者：

Breuker, K ^{[1
]}

Knochenmuss, R ^{[1
]}

Zenobi, R ^{[1
]}

机构：

[1] ETH Zurich, Swiss Fed Inst Technol, Dept Chem, CH-8092 Zurich, Switzerland

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 1999年 / 184卷 / 01期

关键词：

matrix-assisted laser desorption/ionization; matrix; bracketing; gas-phase basicity; oligonucleotides;

D O I：

10.1016/S1387-3806(98)14200-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

The gas-phase basicities (GB) of eight matrix-assisted laser desorption/ionization (MALDI) matrix anions were determined using a modified bracketing method. Reference anions were generated by laser desorption from a newly developed two-phase liquid/solid mixed matrix, allowing measurements at a well defined temperature (299 K) and under low pressure conditions. The most basic matrix anion found was that of 3-aminoquinoline (GB = 1451 kJ/mol) and the least basic was the 2,4,6-trihydroxyacetophenone anion (GB = 1324 kJ/mol). Consequences for the MALDI process are discussed with respect to matrix ion formation, oligonucleotide and protein analysis. Ground-state proton transfer plume reactions between matrix anions and analyte molecules were found to be energetically favorable in many cases. (Int J Mass Spectrom 184 (1999) 25-38) (C) 1999 Elsevier Science B.V.

引用

页码：25 / 38

页数：14