Human phenol sulfotransferase gene contains two alternative promoters: Structure and expression of the gene

被引:25
作者
Bernier, F
Soucy, P
LuuThe, V
机构
[1] CHU LAVAL,RES CTR,MRC,GRP MOL ENDOCRINOL,QUEBEC CITY,PQ G1V 4G2,CANADA
[2] UNIV LAVAL,QUEBEC CITY,PQ G1V 4G2,CANADA
关键词
D O I
10.1089/dna.1996.15.367
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phenol sulfotransferases catalyze the transfer of a sulfonate moiety from 3'-phosphoadenosyl 5'-phosphosulfate to a phenolic group of lipophylic substrates to generate soluble sulfate esters, Using a phenol sulfotransferase cDNA as probe to screen a human leukocyte genomic DNA library constructed in lambda EMBL3, we obtained a clone containing a complete gene sequence, Comparison of the gene sequence with that of the corresponding cDNAs, namely phenol-sulfating phenol sulfotransferase (P-PST) or thermostable sulfotransferase (TS-PST), and human aryl sulfotransferase 1 and 2 (HAST1 and HAST2) indicates that the gene possesses eight short exons separated by seven introns included in approximately 5 kb, HAST2 has a different 5' untranslated sequence, and thus is encoded by a different mRNA species, While the nucleotide sequence corresponding to the 5' noncoding region of P-PST (TS-PST and HAST1) is included in the exon I, the 5' untranslated sequence of HAST2 is located in the beginning of exon IIa. The remaining sequence in exon II that is identical to both P-PST and HAST2 was termed exon IIb. Exons III to VIII, which cover the coding region and the 3' untranslated region, are almost identical in all types of PST or AST cDNAs, These results suggest that the phenol sulfotransferase gene possesses two alternate promoters that drive the expression of the two different mRNA species in a tissue-specific manner, Transfection of chloramphenicol acetyl transferase (CAT) reporter gene vectors containing the 5'-flanking sequence upstream from exon I and exon II, respectively, in transformed human embryonal kidney (293) cells indicate that both sequences possess promoter activity with higher activity for promoter 1. RNA blot analysis indicates that human phenol sulfotransferase gene is expressed in kidney, liver, lung, leukocyte, colon, small intestine, and spleen.
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页码:367 / 375
页数:9
相关论文
共 39 条
  • [31] CHARACTERIZATION OF CDNAS FOR HUMAN ESTRADIOL 17-BETA-DEHYDROGENASE AND ASSIGNMENT OF THE GENE TO CHROMOSOME-17 - EVIDENCE OF 2 MESSENGER-RNA SPECIES WITH DISTINCT 5'-TERMINI IN HUMAN-PLACENTA
    THE, VL
    LABRIE, C
    ZHAO, HF
    COUET, J
    LACHANCE, Y
    SIMARD, J
    LEBLANC, G
    COTE, J
    BERUBE, D
    GAGNE, R
    LABRIE, F
    [J]. MOLECULAR ENDOCRINOLOGY, 1989, 3 (08) : 1301 - 1309
  • [32] THE VL, 1990, MOL ENDOCRINOL, V3, P1301
  • [33] MOLECULAR-CLONING AND FUNCTIONS OF RAT-LIVER HYDROXYSTEROID SULFOTRANSFERASES CATALYZING COVALENT BINDING OF CARCINOGENIC POLYCYCLIC ARYLMETHANOLS TO DNA
    WATABE, T
    OGURA, K
    SATSUKAWA, M
    OKUDA, H
    HIRATSUKA, A
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1994, 92 (1-3) : 87 - 105
  • [34] SULFATION PHARMACOGENETICS IN HUMANS
    WEINSHILBOUM, R
    AKSOY, I
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 1994, 92 (1-3) : 233 - 246
  • [35] WILBORN TW, 1993, MOL PHARMACOL, V43, P70
  • [36] HUMAN LIVER THERMOLABILE PHENOL SULFOTRANSFERASE - CDNA CLONING, EXPRESSION AND CHARACTERIZATION
    WOOD, TC
    AKSOY, IA
    AKSOY, S
    WEINSHILBOUM, RM
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 198 (03) : 1119 - 1127
  • [37] A SINGLE-MOUSE ALPHA-AMYLASE GENE SPECIFIES 2 DIFFERENT TISSUE-SPECIFIC MESSENGER-RNAS
    YOUNG, RA
    HAGENBUCHLE, O
    SCHIBLER, U
    [J]. CELL, 1981, 23 (02) : 451 - 458
  • [38] MOLECULAR CHARACTERIZATION OF A HUMAN ARYL SULFOTRANSFERASE CDNA
    ZHU, XY
    VERONESE, ME
    SANSOM, LN
    MCMANUS, ME
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 192 (02) : 671 - 676
  • [39] IDENTIFICATION OF 2 HUMAN BRAIN ARYL SULFOTRANSFERASE CDNAS
    ZHU, XY
    VERONESE, ME
    BERNARD, CCA
    SANSOM, LN
    MCMANUS, ME
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 195 (01) : 120 - 127