The ssgB gene, encoding a member of the regulon of stress-response sigma factor σH, is essential for aerial mycelium septation in Streptomyces coelicolor A3(2)

被引：8

作者：

Sevcikova, B ^{[1
]}

Kormanec, J ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Mol Biol, Ctr Excellence Mol Med, Bratislava 84551, Slovakia

来源：

ARCHIVES OF MICROBIOLOGY | 2003年 / 180卷 / 05期

关键词：

differentiation; gene disruption; recombinant DNA; RNA polymerase; sigma factor; stress response; Streptomyces coelicolor A3(2);

D O I：

10.1007/s00203-003-0603-y

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Streptomyces coelicolor A3(2) gene ssgB belongs to the regulon of stress-response sigma factor sigma(H). By integrative transformation via double cross-over, a stable null mutant of ssgB was obtained. This mutation had no obvious effect on vegetative growth, but critically affected aerial mycelium septation. The S. coelicolor ssgB mutant produced aerial hyphae without any signs of septation into spore compartments. The mutation was complemented in trans by wild-type ssgB including the sigma(H)-dependent ssgBp promoter. The results proved that ssgB belongs a developmental branch of the sigma(H) regulon.

引用

页码：380 / 384

页数：5

共 17 条

[1]

Ausubel FM, 1995, CURRENT PROTOCOLS MO

[2]

Chater KF, 2000, PROKARYOTIC DEVELOPMENT, P33

[3] DELETION OF DNA LYING CLOSE TO THE GLKA LOCUS INDUCES ECTOPIC SPORULATION IN STREPTOMYCES-COELICOLOR A3(2) [J].

KELEMEN, GH ;

PLASKITT, KA ;

LEWIS, CG ;

FINDLAY, KC ;

BUTTNER, MJ .

MOLECULAR MICROBIOLOGY, 1995, 17 (02) :221-230

[4] A connection between stress and development in the multicellular prokaryote Streptomyces coelicolor A3(2) [J].

Kelemen, GH ;

Viollier, PH ;

Tenor, JL ;

Marri, L ;

Buttner, MJ ;

Thompson, CJ .

MOLECULAR MICROBIOLOGY, 2001, 40 (04) :804-814

[5]

Kieser T., 2000, PRACTICAL STREPTOMYC, DOI DOI 10.1111/J.1365-2427.2007.01876.X

[6] The Streptomyces aureofaciens homologue of the whiB gene is essential for sporulation; Its expression correlates with the developmental stage [J].

Kormanec, J ;

Sevcikova, B ;

Sprusansky, O ;

Benada, O ;

Kofronova, O ;

Novakova, R ;

Rezuchova, B ;

Potuckova, L ;

Homerova, D .

FOLIA MICROBIOLOGICA, 1998, 43 (06) :605-612

[7] Stress-response sigma factor σH directs expression of the gltB gene encoding glutamate synthase in Streptomyces coelicolor A3(2) [J].

Kormanec, J ;

Sevcikova, B .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2002, 1577 (01) :149-154

[8] The stress-response sigma factor σH controls the expression of ssgB, a homologue of the sporulation-specific cell division gene ssgA, in Streptomyces coelicolor A3(2) [J].

Kormanec, J ;

Sevcikova, B .

MOLECULAR GENETICS AND GENOMICS, 2002, 267 (04) :536-543

[9] Identification and transcriptional characterization of the gene encoding the stress-response σ factor σH in Streptomyces coelicolor A3(2) [J].

Kormanec, J ;

Sevcíková, B ;

Halgasová, N ;

Knirschová, R ;

Rezuchová, B .

FEMS MICROBIOLOGY LETTERS, 2000, 189 (01) :31-38

[10] ANALYSIS OF STREPTOMYCES-AVERMITILIS GENES REQUIRED FOR AVERMECTIN BIOSYNTHESIS UTILIZING A NOVEL INTEGRATION VECTOR [J].

MACNEIL, DJ ;

GEWAIN, KM ;

RUBY, CL ;

DEZENY, G ;

GIBBONS, PH ;

MACNEIL, T .

GENE, 1992, 111 (01) :61-68

← 1 2 →