Ratiometric Temperature Sensing with Semiconducting Polymer Dots

被引：349

作者：

Ye, Fangmao ^{[1
]}

Wu, Changfeng ^{[1
]}

Jin, Yuhui ^{[1
]}

Chan, Yang-Hsiang ^{[1
]}

Zhang, Xuanjun ^{[1
]}

Chiu, Daniel T. ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 21期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

DEPENDENT FLUORESCENT DYE; NANOPARTICLES; THERMOMETRY; ULTRABRIGHT; LIFETIME; SENSORS;

D O I：

10.1021/ja202945g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye-Rhodamine B (RhB), whose emission intensity decreases with increasing temperature-within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.

引用

页码：8146 / 8149

页数：4

共 22 条

[1] OPTICAL-FIBER TEMPERATURE SENSOR BASED ON UPCONVERSION-EXCITED FLUORESCENCE [J].