Hydrophilic Fluorescent Nanogel Thermometer for Intracellular Thermometry

被引：455

作者：

Gota, Chie ^{[1
]}

Okabe, Kohki ^{[1
,2
]}

Funatsu, Takashi ^{[1
,3
]}

Harada, Yoshie ^{[2
]}

Uchiyama, Seiichi ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Tokyo Metropolitan Inst Med Sci, Bunkyo Ku, Tokyo 1138613, Japan

[3] Univ Tokyo, Ctr NanoBio Integrat, Bunkyo Ku, Tokyo 1138656, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 08期

关键词：

SINGLE LIVING CELLS; TEMPERATURE-MEASUREMENT; PHASE-TRANSITIONS; THERMOGENESIS; MICROGELS; POLYMERS; POLARITY; PROBES; VOLUME; HEAT;

D O I：

10.1021/ja807714j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The first methodology to measure intracellular temperature is described. A highly hydrophilic fluorescent nanogel thermometer developed for this purpose stays in the cytoplasm and emits stronger fluorescence at a higher temperature. Thus, intracellular temperature variations associated with biological processes can be monitored by this novel thermometer with a temperature resolution of better than 0.5 degrees C.

引用

页码：2766 / +

页数：3

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