Mammalian homologues of yeast vacuolar protein sorting (vps) genes implicated in Golgi-to-lysosome trafficking

被引:46
作者
Pevsner, J [1 ]
Hsu, SC [1 ]
Hyde, PS [1 ]
Scheller, RH [1 ]
机构
[1] STANFORD UNIV, HOWARD HUGHES MED INST, DEPT CELLULAR & MOL PHYSIOL, BECKMAN CTR, STANFORD, CA 94305 USA
关键词
vesicles; secretion; n-sec1; vacuole; syntaxin;
D O I
10.1016/S0378-1119(96)00367-8
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Sec1p, Vps33p, Vps45p and Sly1p constitute a family of proteins implicated in vesicle trafficking at distinct stages of the yeast secretory pathway. Several mammalian homologues of Sec1p have been described, including n-sec1 which has been implicated in the regulation of synaptic vesicle docking at the nerve terminal. We have characterized cDNA clones encoding three additional mammalian homologues belonging to this family: r-vps33a and r-vps33b from rat, which are 30 and 26% identical to yeast Vps33p, respectively, and h-vps45 from human which is 38% identical to yeast Vps45p at the amino acid (aa) level. Phylogenetic analysis of 16 Sec1p-related proteins from several species is consistent with the hypothesis that the evolution of this gene family parallels the specialization of vesicle trafficking to distinct intracellular compartments. By Northern analysis, each of these genes is expressed in all tissues examined (brain, spleen, lung, liver, skeletal muscle, kidney, testis). While n-sec1 binds syntaxin 1a, 2, and 3, r-vps33a, r-vps33b and h-vps45 do not bind any of the known syntaxins. We propose that the three proteins bind as yet unidentified syntaxin homologues involved in vesicle trafficking between the Golgi apparatus, prelysosomal compartment(s), and the lysosome.
引用
收藏
页码:7 / 14
页数:8
相关论文
共 30 条
  • [11] RBSEC1A AND RBSEC1B COLOCALIZE WITH SYNTAXIN-1 AND SNAP-25 THROUGHOUT THE AXON, BUT ARE NOT IN A STABLE COMPLEX WITH SYNTAXIN
    GARCIA, EP
    MCPHERSON, PS
    CHILCOTE, TJ
    TAKEI, K
    DECAMILLI, P
    [J]. JOURNAL OF CELL BIOLOGY, 1995, 129 (01) : 105 - 120
  • [12] A NOVEL UBIQUITOUS FORM OF MUNC-18 INTERACTS WITH MULTIPLE SYNTAXINS - USE OF THE YEAST 2-HYBRID SYSTEM TO STUDY INTERACTIONS BETWEEN PROTEINS INVOLVED IN MEMBRANE TRAFFIC
    HATA, Y
    SUDHOF, TC
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (22) : 13022 - 13028
  • [13] SYNAPTIC VESICLE FUSION COMPLEX CONTAINS UNC-18 HOMOLOG BOUND TO SYNTAXIN
    HATA, Y
    SLAUGHTER, CA
    SUDHOF, TC
    [J]. NATURE, 1993, 366 (6453) : 347 - 351
  • [14] AN EMPIRICAL-TEST OF BOOTSTRAPPING AS A METHOD FOR ASSESSING CONFIDENCE IN PHYLOGENETIC ANALYSIS
    HILLIS, DM
    BULL, JJ
    [J]. SYSTEMATIC BIOLOGY, 1993, 42 (02) : 182 - 192
  • [15] HODEL A, 1994, J BIOL CHEM, V269, P8623
  • [16] A NOVEL ISOFORM OF SYNTAXIN-BINDING PROTEIN HOMOLOGOUS TO YEAST SEC1 EXPRESSED UBIQUITOUSLY IN MAMMALIAN-CELLS
    KATAGIRI, H
    TERASAKI, J
    MURATA, T
    ISHIHARA, H
    OGIHARA, T
    INUKAI, K
    FUKUSHIMA, Y
    ANAI, M
    KIKUCHI, M
    MIYAZAKI, J
    YAZAKI, Y
    OKA, Y
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (10) : 4963 - 4966
  • [17] THE SCANNING MODEL FOR TRANSLATION - AN UPDATE
    KOZAK, M
    [J]. JOURNAL OF CELL BIOLOGY, 1989, 108 (02) : 229 - 241
  • [18] PROTEIN PROCESSING IN LYSOSOMES - THE NEW THERAPEUTIC TARGET IN NEURODEGENERATIVE DISEASE
    MAYER, RJ
    LANDON, M
    LASZLO, L
    LENNOX, G
    LOWE, J
    [J]. LANCET, 1992, 340 (8812) : 156 - 159
  • [19] NOTHWEHR SF, 1994, J BIOL CHEM, V269, P10185
  • [20] IDENTIFICATION OF 23 COMPLEMENTATION GROUPS REQUIRED FOR POST-TRANSLATIONAL EVENTS IN THE YEAST SECRETORY PATHWAY
    NOVICK, P
    FIELD, C
    SCHEKMAN, R
    [J]. CELL, 1980, 21 (01) : 205 - 215