Purification and characterization of cannabichromenic acid synthase from Cannabis sativa

被引：88

作者：

Morimoto, S ^{[1
]}

Komatsu, K ^{[1
]}

Taura, F ^{[1
]}

Shoyama, Y ^{[1
]}

机构：

[1] Kyushu Univ, Fac Pharmaceut Sci, Higashi Ku, Fukuoka 81282, Japan

来源：

PHYTOCHEMISTRY | 1998年 / 49卷 / 06期

关键词：

Cannabis sativa; Moraceae; biosynthesis; cannabichromenic acid synthase; cannabichromenic acid; cannabigerolic acid;

D O I：

10.1016/S0031-9422(98)00278-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cannabichromenic acid synthase was purified to:apparent homogeneity by sequential column chromatography including DEAE-cellulose, phenyl-Sepharose CL-4B, and hydroxylapatite. The enzyme catalysed the oxidocyclization of cannabigerolic acid and cannabinerolic acid to cannabichromenic acid. The K-m values for both substrates were in the same order of magnitude although the V-max value for the former was higher than that for the latter. These results suggested that cannabichromenic acid is predominantly formed from cannabigerolic acid rather than cannabinerolic acid. The enzyme required neither molecular oxygen nor hydrogen peroxide, indicating that the cannabichromenic acid synthase reaction proceeds through direct dehydrogenation without hydroxylation. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1525 / 1529

页数：5

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