Description and evaluation of a short-term reproduction test with the fathead minnow (Pimephales promelas)

被引:349
作者
Ankley, GT [1 ]
Jensen, KM [1 ]
Kahl, MD [1 ]
Korte, JJ [1 ]
Makynen, EA [1 ]
机构
[1] US EPA, Off Res & Dev, Natl Hlth & Environm Effects Res Lab, Midcontinent Ecol Div, Duluth, MN 55804 USA
关键词
fathead minnow; reproduction; endocrine function; toxicity;
D O I
10.1002/etc.5620200616
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Due to the time and expense associated with full life-cycle testing, most current toxicity tests with fish do not explicitly consider reproductive output as an endpoint but, rather, focus on early life-stage survival and development. However, some classes of chemicals could adversely impact reproduction at concentrations below those that affect development, Further, estimates of the effects of toxic compounds on reproductive output can be critical to the ecological risk assessment process. In this manuscript, we describe a short-term reproduction test with the fathead minnow (Pimephales promelas) and evaluate the test using two model reproductive toxicants, methoxychlor (an estrogenic compound) and methyltestosterone (an androgenic chemical). The test is initiated with reproductively mature animals and is comprised of a pre-exposure phase of 14 to 21 d, followed by a chemical exposure of up to 21 d. During and at completion of the test, several endpoints related to reproductive fitness and endocrine function are assessed, Both chemicals evaluated in our study caused a significant decrease in fecundity of the fish at nominal concentrations of 5.0 mug/L (methoxychlor) and 0.2 mg/L (methyltestosterone). Methoxychlor decreased plasma concentrations of one or more steroids (testosterone, 11-ketotestosterone, beta -estradiol) in both sexes and caused a significant induction of plasma vitellogenin in males. a response consistent with activation of the estrogen receptor by the pesticide (or its metabolites). Methyltebtosterone decreased plasma concentrations of sex steroids and adversely affected gonadal status (as evaluated by relative weight and histopathology) in both sexes. The androgenic nature of methyltestosterone was clearly expressed as masculinization of exposed females via formation of nuptial tubercles, structures normally present only in reproductively active males. The chemical also caused a significant induction of plasma vitellogenin in both males and females; this unexpected estrogenic response was most likely due to aromatization of the androgen to a form capable of binding to the estrogen receptor. These studies demonstrate the utility of this short-term assay for identifying chemicals that exert reproductive toxicity through alterations in endocrine systems controlled by estrogens and androgens.
引用
收藏
页码:1276 / 1290
页数:15
相关论文
共 81 条
  • [31] Aspects of basic reproductive biology and endocrinology in the fathead minnow (Pimephales promelas)
    Jensen, KM
    Korte, JJ
    Kahl, MD
    Pasha, MS
    Ankley, GT
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 2001, 128 (01): : 127 - 141
  • [32] Saturation units for use in aquatic bioassays
    Kahl, MD
    Russom, CL
    DeFoe, DL
    Hammermeister, DE
    [J]. CHEMOSPHERE, 1999, 39 (03) : 539 - 551
  • [33] Kavlock RJ, 1996, ENVIRON HEALTH PERSP, V104, P715, DOI 10.1289/ehp.96104s4715
  • [34] Kime D., 1998, Endocrine disruption in fish, DOI 10.1007/978-1-4615-4943-7
  • [35] Fathead minnow vitellogenin:: Complementary DNA sequence and messenger RNA and protein expression after 17β-estradiol treatment
    Korte, JJ
    Kahl, MD
    Jensen, KM
    Pasha, MS
    Parks, LG
    LeBlanc, GA
    Ankley, GT
    [J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2000, 19 (04) : 972 - 981
  • [36] Reproductive impairment and induction of alkaline-labile phosphate, a biomarker of estrogen exposure, in fathead minnows (Pimephales promelas) exposed to waterborne 17β-estradiol
    Kramer, VJ
    Miles-Richardson, S
    Pierens, SL
    Giesy, JP
    [J]. AQUATIC TOXICOLOGY, 1998, 40 (04) : 335 - 360
  • [37] Exposure of juvenile rainbow trout (Oncorhynchus mykiss) to methoxychlor results in a dose-dependent decrease in growth and survival but does not alter male sexual differentiation
    Krisfalusi, M
    Eroschenko, VP
    Cloud, JG
    [J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1998, 60 (04) : 659 - 666
  • [38] EFFECTS OF MIREX AND METHOXYCHLOR ON JUVENILE AND ADULT STRIPED MULLET, MUGIL-CEPHALUS L
    LEE, JH
    SYLVESTER, JR
    NASH, CE
    [J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1975, 14 (02) : 180 - 186
  • [39] Lewis P.A., 1994, SHORT TERM METHODS E
  • [40] Effects of the mammalian antiandrogen vinclozolin on development and reproduction of the fathead minnow (Pimephales promelas)
    Makynen, EA
    Kahl, MD
    Jensen, KM
    Tietge, JE
    Wells, KL
    Van der Kraak, G
    Ankley, GT
    [J]. AQUATIC TOXICOLOGY, 2000, 48 (04) : 461 - 475