Short Report: Evaluation of Loop-Mediated Isothermal Amplification (LAMP) for Malaria Diagnosis in a Field Setting

被引：64

作者：

Sirichaisinthop, Jeeraphat ^{[2
]}

Buates, Sureemas ^{[1
]}

Watanabe, Risa ^{[3
]}

Han, Eun-Taek ^{[4
]}

Suktawonjaroenpon, Wachira ^{[5
]}

Krasaesub, Somporn

Takeo, Satoru ^{[3
]}

Tsuboi, Takafumi ^{[3
,6
]}

Sattabongkot, Jetsumon ^{[7
]}

机构：

[1] Mahidol Univ, Dept Microbiol, Fac Sci, Bangkok 10400, Thailand

[2] Vector Borne Dis Training Ctr, Pra Budhabat 18120, Saraburi, Thailand

[3] Ehime Univ, Cell Free Sci & Technol Res Ctr, Matsuyama, Ehime 7908577, Japan

[4] Kangwon Natl Univ, Coll Med, Dept Parasitol, Chunchon 200701, South Korea

[5] Armed Forces Res Inst Med Sci, Dept Entomol, Bangkok 10400, Thailand

[6] Ehime Univ, Venture Business Lab, Matsuyama, Ehime 7908577, Japan

[7] Mahidol Univ, Mahidol Vivax Res Ctr, Fac Trop Med, Bangkok 10400, Thailand

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2011年 / 85卷 / 04期

关键词：

POLYMERASE-CHAIN-REACTION; PLASMODIUM-FALCIPARUM; THAILAND; INFECTIONS; PARASITES; AREA; PCR;

D O I：

10.4269/ajtmh.2011.10-0676

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers for the four human malaria parasites at a field clinic in comparison with standard microscopy. Among 110 blood samples collected from the malaria clinic in Thailand, LAMP detected 59 of 60 samples positive by microscopy (sensitivity = 98.3%) and none of the 50 microscopy-negative samples (specificity = 100%). Negative predictive value (NPV) and positive predictive value (PPV) of LAMP were 98% and 100%, respectively. These results indicate that LAMP is an effective tool for malaria diagnosis at a field clinic in a field setting.

引用

页码：594 / 596

页数：3

共 15 条

[1] Dynamics of gametocytes among Plasmodium falciparum clones in natural infections in an area of highly seasonal transmission [J].

Abdel-Wahab, A ;

Abdel-Muhsin, AMA ;

Ali, E ;

Suleiman, S ;

Ahmed, S ;

Walliker, D ;

Babiker, HA .

JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (12) :1838-1842

[2] Comparison of PCR and microscopy for the detection of asymptomatic malaria in a Plasmodium falciparum/vivax endemic area in Thailand [J].

Coleman, Russell E. ;

Sattabongkot, Jetsumon ;

Promstaporm, Sommai ;

Maneechai, Nongnuj ;

Tippayachai, Bousaraporn ;

Kengluecha, Ampornpan ;

Rachapaew, Nattawan ;

Zollner, Gabriela ;

Miller, Robert Scott ;

Vaughan, Jefferson A. ;

Thimasarn, Krongtong ;

Khuntirat, Benjawan .

MALARIA JOURNAL, 2006, 5 (1)

[3] Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis [J].

Han, Eun-Taek ;

Watanabe, Risa ;

Sattabongkot, Jetsumon ;

Khuntirat, Benjawan ;

Sirichaisinthop, Jeeraphat ;

Iriko, Hideyuki ;

Jin, Ling ;

Takeo, Satoru ;

Tsuboi, Takafumi .

JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (08) :2521-2528

[4] How useful is PCR in the diagnosis of malaria? [J].

Hänscheid, T ;

Grobusch, MP .

TRENDS IN PARASITOLOGY, 2002, 18 (09) :395-398

[5]

Kimura Masatsugu, 1997, Parasitology International, V46, P91, DOI 10.1016/S1383-5769(97)00013-5

[6] Rapid diagnostic tests for malaria parasites [J].

Moody, A .

CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (01) :66-+

[7] Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation [J].

Mori, Y ;

Nagamine, K ;

Tomita, N ;

Notomi, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 289 (01) :150-154

[8] Update on rapid diagnostic testing for malaria [J].

Murray, Clinton K. ;

Gasser, Robert A., Jr. ;

Magill, Alan J. ;

Miller, R. Scott .

CLINICAL MICROBIOLOGY REVIEWS, 2008, 21 (01) :97-+

[9] Current malaria status and distribution of drug resistance in East and Southeast Asia with special focus to Thailand [J].

Na-Bangchang, Kesara ;

Congpuong, Kanungnit .

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2007, 211 (02) :99-113

[10]

Nagamine K, 2001, CLIN CHEM, V47, P1742

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