Global profiling of DNA methylation erasure in mouse primordial germ cells

被引：264

作者：

Guibert, Sylvain ^{[1
,2
]}

Forne, Thierry ^{[1
]}

Weber, Michael ^{[1
,2
]}

机构：

[1] Univ Montpellier I, Univ Montpellier 2, Inst Mol Genet, CNRS,UMR 5535, F-34293 Montpellier 5, France

[2] Univ Strasbourg, CNRS, ESBS, UMR Biotechnol & Cell Signalling 7242, F-67412 Illkirch Graffenstaden, France

来源：

GENOME RESEARCH | 2012年 / 22卷 / 04期

关键词：

EPIGENETIC INHERITANCE; DYNAMICS; GENE; CHROMATIN; SPECIFICATION; PLURIPOTENT; EXPRESSION; MECHANISM; LINEAGE; EMBRYOS;

D O I：

10.1101/gr.130997.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epigenetic reprogramming, characterized by loss of cytosine methylation and histone modifications, occurs during mammalian development in primordial germ cells (PGCs), yet the targets and kinetics of this process are poorly characterized. Here we provide a map of cytosine methylation on a large portion of the genome in developing male and female PGCs isolated from mouse embryos. We show that DNA methylation erasure is global and affects genes of various biological functions. We also reveal complex kinetics of demethylation that are initiated at most genes in early PGC precursors around embryonic day 8.0-9.0. In addition, besides intracisternal A-particles (IAPs), we identify rare LTR-ERVI retroelements and single-copy sequences that resist global methylation erasure in PGCs as well as in preimplantation embryos. Our data provide important insights into the targets and dynamics of DNA methylation reprogramming in mammalian germ cells.

引用

页码：633 / 641

页数：9

共 36 条

[1] Targets and dynamics of promoter DNA methylation during early mouse development [J].

Borgel, Julie ;

Guibert, Sylvain ;

Li, Yufeng ;

Chiba, Hatsune ;

Schuebeler, Dirk ;

Sasaki, Hiroyuki ;

Forne, Thierry ;

Weber, Michael .

NATURE GENETICS, 2010, 42 (12) :1093-U90

[2] Tet1 Is Dispensable for Maintaining Pluripotency and Its Loss Is Compatible with Embryonic and Postnatal Development [J].

Dawlaty, Meelad M. ;

Ganz, Kibibi ;

Powell, Benjamin E. ;

Hu, Yueh-Chiang ;

Markoulaki, Styliani ;

Cheng, Albert W. ;

Gao, Qing ;

Kim, Jongpil ;

Choi, Sang-Woon ;

Page, David C. ;

Jaenisch, Rudolf .

CELL STEM CELL, 2011, 9 (02) :166-175

[3] Epigenetic Reprogramming in Plant and Animal Development [J].

Feng, Suhua ;

Jacobsen, Steven E. ;

Reik, Wolf .

SCIENCE, 2010, 330 (6004) :622-627

[4] DNA methyltransferases control telomere length and telomere recombination in mammalian cells [J].

Gonzalo, S ;

Jaco, I ;

Fraga, MF ;

Chen, TP ;

Li, E ;

Esteller, M ;

Blasco, MA .

NATURE CELL BIOLOGY, 2006, 8 (04) :416-U66

[5] Dynamic regulation of DNA methylation during mammalian development [J].

Guibert, Sylvain ;

Forne, Thierry ;

Weber, Michael .

EPIGENOMICS, 2009, 1 (01) :81-98

[6] Epigenetic reprogramming in mouse primordial germ cells [J].

Hajkova, P ;

Erhardt, S ;

Lane, N ;

Haaf, T ;

El-Maarri, O ;

Reik, W ;

Walter, J ;

Surani, MA .

MECHANISMS OF DEVELOPMENT, 2002, 117 (1-2) :15-23

[7] Chromatin dynamics during epigenetic reprogramming in the mouse germ line [J].

Hajkova, Petra ;

Ancelin, Katia ;

Waldmann, Tanja ;

Lacoste, Nicolas ;

Lange, Ulrike C. ;

Cesari, Francesca ;

Lee, Caroline ;

Almouzni, Genevieve ;

Schneider, Robert ;

Surani, M. Azim .

NATURE, 2008, 452 (7189) :877-U6

[8] Genome-Wide Reprogramming in the Mouse Germ Line Entails the Base Excision Repair Pathway [J].

Hajkova, Petra ;

Jeffries, Sean J. ;

Lee, Caroline ;

Miller, Nigel ;

Jackson, Stephen P. ;

Surani, M. Azim .

SCIENCE, 2010, 329 (5987) :78-82

[9] Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources [J].

Huang, Da Wei ;

Sherman, Brad T. ;

Lempicki, Richard A. .

NATURE PROTOCOLS, 2009, 4 (01) :44-57

[10] Environmental epigenomics and disease susceptibility [J].

Jirtle, Randy L. ;

Skinner, Michael K. .

NATURE REVIEWS GENETICS, 2007, 8 (04) :253-262

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