Amperometric immunosensors based on layer-by-layer assembly of gold nanoparticles and methylene blue on thiourea modified glassy carbon electrode for determination of human chorionic gonadotrophin

被引：79

作者：

Chai, Rong ^{[1
]}

Yuan, Ruo ^{[1
]}

Chai, Yaqin ^{[1
]}

Ou, Chaofeng ^{[1
]}

Cao, Shurui ^{[1
]}

Li, Xuelian ^{[1
]}

机构：

[1] SW Univ, Coll Chem & Chem Engn, Chongqing Key Lab, Chongqing 400715, Peoples R China

来源：

TALANTA | 2008年 / 74卷 / 05期

基金：

中国国家自然科学基金;

关键词：

immunosensor; thiourea; layer-by-layer (LBL); gold nanoparticles (nano-Au); human chorionic gonadotrophin;

D O I：

10.1016/j.talanta.2007.08.046

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nano-Au and methylene blue (MB) were assembled layer-by-layer (LBL) into films on the glassy carbon electrode modified by thiourea for detection of human chorionic gonadotrophin (HCG). The electrode modification process was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron micrograph (SEM). The factors influencing the performance of the amperometric immunosensor were studied in detail. Tests performed with this immunosensor showed good linearity, the working range for the system was 1.0-100.0 mIU/mL with a detection limit of 0.3 mIU/mL at 3 sigma. Moreover, the studied immunosensor exhibited high sensitivity and long-term stability. The present work supplied a promising test method for clinical immunoassay. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1330 / 1336

页数：7

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