A reusable capacitive immunosensor for carcinoembryonic antigen (CEA) detection using thiourea modified gold electrode

被引：91

作者：

Limbut, W

Kanatharana, P

Mattiasson, B

Asawatreratanakul, P

Thavarungkul, P ^{[1
]}

机构：

[1] Prince Songkla, Biophys Res Unit Biosensors & Biocurrents, Songkhla 90112, Thailand

[2] Prince Songkla, Fac Sci, Dept Chem, Songkhla 90112, Thailand

[3] Lund Univ, Ctr Chem & Chem Engn, Dept Biotechnol, S-22100 Lund, Sweden

[4] Prince Songkla, Fac Sci, Dept Biochem, Songkhla 90112, Thailand

[5] Prince Songkla, Fac Sci, Dept Phys, Songkhla 90112, Thailand

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 561卷 / 1-2期

关键词：

capacitance; immunosensor; carcinoembryonic antigen; thiourea; self-assembled monolayer;

D O I：

10.1016/j.aca.2006.01.021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A capacitive immunosensor based on a self-assembled monolayer (SAM) of thiourea on gold electrode has been developed. Anti-carcinoembryonic antigen (anti-CEA) was immobilized on a self-assembled thiourea monolayer (SATUM) via covalent coupling. Under optimum conditions, the decrease in capacitive signal when carcinoembryonic antigen (CEA) standard was injected could be determined with a detection limit of 10 pg ml(-1) and linearity in the range of 0.01-10 ng ml(-1). The immobilized anti-CEA on SATUM gold electrode was stable and after regeneration good reproducibility of the signal could be obtained tip to 45 times with an RSD lower than 3.4%. Good agreement was obtained when CEA concentrations of human serum samples determined by the flow injection capacitive immunosensor system were compared to those obtained using an enzyme linked fluorescent assay (ELFA) method (P < 0.05). (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：55 / 61

页数：7

共 30 条

[1] FORMATION OF MONOLAYER FILMS BY THE SPONTANEOUS ASSEMBLY OF ORGANIC THIOLS FROM SOLUTION ONTO GOLD [J].