The label free picomolar detection of insulin in blood serum

被引:123
作者
Xu, Mengyun [1 ]
Luo, Xiliang [1 ,2 ]
Davis, Jason J. [1 ]
机构
[1] Univ Oxford, Dept Chem, Oxford OX1 3QZ, England
[2] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Biochem Anal, Qingdao 266042, Peoples R China
关键词
Insulin; Electrochemical impedance spectroscopy; Biosensor; Diabetes; Blood serum; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; SELF-ASSEMBLED MONOLAYERS; FLUORESCENCE DETECTION; MODIFIED ELECTRODE; OXIDATION; PROTEIN; SENSOR; OXIDE; RADIOIMMUNOASSAY; DISSOCIATION;
D O I
10.1016/j.bios.2012.06.014
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Insulin, a polypeptide hormone secreted by pancreatic cells, is a key regulator in glucose homeostasis. Its deficiency leads to insulin-dependent (type I) diabetes whereas resistance to insulin is common in type II diabetes, obesity and a range of endocrine disorders. Its determination is of considerable value, particularly in the clinical diagnosis of diabetes mellitus and the doping control of athletes. It has, additionally, been noted as a potential breast cancer marker (serum insulin levels being found to be raised in comparison to control patients). Electrochemical assays are potentially very cheap, highly sensitive, and very readily transposed to a point of care. Though there exist numerous examples of label free impedimetric or capacitative assaying of biomolecules, these are rarely demonstrated to be effective in complex biological mixtures or to be applicable to low molecular weight targets (since they operate through the interfacial displacement of water/ions and/or the steric blocking of a redox probe). We report herein an ultrasensitive electrochemical and label-free biosensor for insulin in blood serum with a clinically relevant linear range and detection limit of 1.2 pM. The transducing surfaces, based on readily prepared, antibody modified, polyethylene glycol monolayer modified polycrystalline gold surfaces, respond in a highly specific and re-useable manner to the target in up to 50% blood serum. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:21 / 25
页数:5
相关论文
共 43 条
[1]   Electrocatalytic oxidation and determination of insulin at CNT-nickel-cobalt oxide modified electrode [J].
Arvinte, Adina ;
Westermann, A. Caroline ;
Sesay, Adama Marie ;
Virtanen, Vesa .
SENSORS AND ACTUATORS B-CHEMICAL, 2010, 150 (02) :756-763
[2]   Rapid insulin assay for intraoperative confirmation of. complete resection of insulinomas [J].
Carneiro, DM ;
Levi, JU ;
Irvin, GL .
SURGERY, 2002, 132 (06) :937-942
[3]   Synthesis of Biocompatible PEG-Based Star Polymers with Cationic and Degradable Core for siRNA Delivery [J].
Cho, Hong Y. ;
Srinivasan, Abiraman ;
Hong, Joanna ;
Hsu, Eric ;
Liu, Shiguang ;
Shrivats, Arun ;
Kwak, Dan ;
Bohaty, Andrew K. ;
Paik, Hyun-jong ;
Hollinger, Jeffrey O. ;
Matyjaszewski, Krzysztof .
BIOMACROMOLECULES, 2011, 12 (10) :3478-3486
[4]   Development of immunosensors for direct detection of three wound infection biomarkers at point of care using electrochemical impedance spectroscopy [J].
Ciani, Ilenia ;
Schulze, Holger ;
Corrigan, Damion K. ;
Henihan, Grace ;
Giraud, Gerard ;
Terry, Jonathan G. ;
Walton, Anthony J. ;
Pethig, Ronald ;
Ghazal, Peter ;
Crain, Jason ;
Campbell, Colin J. ;
Bachmann, Till T. ;
Mount, Andrew R. .
BIOSENSORS & BIOELECTRONICS, 2012, 31 (01) :413-418
[5]   FLOW-INJECTION AMPEROMETRIC DETERMINATION OF INSULIN BASED UPON ITS OXIDATION AT A MODIFIED ELECTRODE [J].
COX, JA ;
GRAY, TJ .
ANALYTICAL CHEMISTRY, 1989, 61 (21) :2462-2464
[6]   Label-free impedance biosensors: Opportunities and challenges [J].
Daniels, Jonathan S. ;
Pourmand, Nader .
ELECTROANALYSIS, 2007, 19 (12) :1239-1257
[7]  
Davis J, 2009, ENGINEERING THE BIOELECTRONIC INTERFACE: APPLICATIONS TO ANALYTE BIOSENSING AND PROTEIN DETECTION, P1, DOI 10.1039/9781847559777
[8]  
Deberg M, 1998, CLIN CHEM, V44, P1504
[9]   Multifunctional Au Nanoparticle Dendrimer-Based Surface Plasmon Resonance Biosensor and Its Application for Improved Insulin Detection [J].
Frasconi, Marco ;
Tortolini, Cristina ;
Botre, Francesco ;
Mazzei, Franco .
ANALYTICAL CHEMISTRY, 2010, 82 (17) :7335-7342
[10]   Self-assembled PEG monolayer based SPR immunosensor for label-free detection of insulin [J].
Gobi, K. Vengatajalabathy ;
Iwasaka, Hiroyuki ;
Miura, Norio .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) :1382-1389