Enumeration of viable Listeria monocytogenes cells by real-time PCR with propidium monoazide and ethidium monoazide in the presence of dead cells

被引：224

作者：

Pan, Y. ^{[1
]}

Breidt, F., Jr. ^{[1
]}

机构：

[1] N Carolina State Univ, USDA ARS, Dept Microbiol, N Carolina Agr Res Serv, Raleigh, NC 27695 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 24期

关键词：

D O I：

10.1128/AEM.01198-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Propidium monoazide (PMA) and ethidium monoazide were used for enumeration of viable Listeria monocytogenes cells in the presence of dead cells. PMA had no antimicrobial effect on L. monocytogenes. Viable cell counts were linearly related to real-time PCR threshold cycle values for PMA-treated cells from planktonic and biofilm sources over a 4-log range.

引用

页码：8028 / 8031

页数：4

共 16 条

[1] BOLTON PH, 1978, NUCLEIC ACIDS RES, V5, P4891
[2] Differentiation of the major Listeria monocytogenes serovars by multiplex PCR
Doumith, M
Buchrieser, C
Glaser, P
Jacquet, C
Martin, P
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) : 3819 - 3822
[3] Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR
Guilbaud, M
de Coppet, P
Bourion, F
Rachman, C
Prévost, H
Dousset, X
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (04) : 2190 - 2194
[4] Detection and quantification of the iap gene of Listeria monocytogenes and Listeria innocua by a new real-time quantitative PCR assay
Hein, I
Klein, D
Lehner, A
Bubert, A
Brandl, E
Wagner, M
[J]. RESEARCH IN MICROBIOLOGY, 2001, 152 (01) : 37 - 46
[5] Rapid enumeration of Listeria monocytogenes in artificially contaminated cabbage using real-time polymerase chain reaction
Hough, AJ
Harbison, SA
Savill, MG
Melton, LD
Fletcher, G
[J]. JOURNAL OF FOOD PROTECTION, 2002, 65 (08) : 1329 - 1332
[6] The cell membrane plays a crucial role in survival of bacteria and archaea in extreme environments
Konings, WN
Albers, SV
Koning, S
Driessen, AJM
[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2002, 81 (1-4): : 61 - 72
[7] Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide
Nocker, A
Camper, AK
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (03) : 1997 - 2004
[8] Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells
Nocker, Andreas
Cheung, Ching-Ying
Camper, Anne K.
[J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2006, 67 (02) : 310 - 320
[9] Application of 5′-nuclease PCR for quantitative detection of Listeria monocytogenes in pure cultures, water, skim milk, and unpasteurized whole milk
Nogva, HK
Rudi, K
Naterstad, K
Holck, A
Lillehaug, D
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) : 4266 - 4271
[10] Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5′-nuclease PCR
Nogva, HK
Dromtorp, SM
Nissen, H
Rudi, K
[J]. BIOTECHNIQUES, 2003, 34 (04) : 804 - +

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