Colony filtration blot:: a new screening method for soluble protein expression in Escherichia coli

被引：55

作者：

Cornvik, T

Dahlroth, SL

Magnusdottir, A

Herman, MD

Knaust, R

Ekberg, M ^{[1
]}

Nordlund, P

机构：

[1] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

[2] Univ Stockholm, Dept Biochem & Biophys, S-10691 Stockholm, Sweden

来源：

NATURE METHODS | 2005年 / 2卷 / 07期

关键词：

D O I：

10.1038/NMETH767

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The implementation of efficient technologies for the production of recombinant mammalian proteins remains an outstanding challenge in many structural and functional genomics programs. We have developed a new method for rapid identification of soluble protein expression in E. coli, based on a separation of soluble protein from inclusion bodies by a filtration step at the colony level. The colony filtration (CoFi) blot is very well suited to screen libraries, and in the present work we used it to screen a deletion mutagenesis library.

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页码：507 / 509

页数：3

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