Fluorescence depletion mechanisms in super-resolving STED microscopy

被引：61

作者：

Rittweger, E. ^{[1
]}

Rankin, B. R. ^{[1
]}

Westphal, V. ^{[1
]}

Hell, S. W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, Gottingen, Germany

来源：

CHEMICAL PHYSICS LETTERS | 2007年 / 442卷 / 4-6期

关键词：

D O I：

10.1016/j.cplett.2007.06.017

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We prove that stimulated emission is by far the dominant quenching mechanism for providing super-resolution in fluorescence microscopy with a red-shifted depletion beam. Our evidences are based on simultaneously measuring fluorescence quenching and photon gain in the quenching beam. Measurements were performed for several fluorescent dyes including fluorescent proteins over a wide spectral range of their emission spectra. We found that, for each fluorophore, the wavelength dependence of both signals closely follows that of the stimulated emission cross-section. (c) 2007 Published by Elsevier B.V.

引用

页码：483 / 487

页数：5

共 17 条

[1]

ALEKSEEV VA, 1979, J APPL SPECTROSC, V31, P51

[2] Ultrafast gain dynamics of the green fluorescent protein [J].

Didier, P ;

Guidoni, L ;

Schwalbach, G ;

Bourotte, M ;

Follenius-Wund, A ;

Pigault, C ;

Bigot, JY .

CHEMICAL PHYSICS LETTERS, 2002, 364 (5-6) :503-510

[3] Macromolecular-scale resolution in biological fluorescence microscopy [J].

Donnert, Gerald ;

Keller, Jan ;

Medda, Rebecca ;

Andrei, M. Alexandra ;

Rizzoli, Silvio O. ;

Luehrmann, Reinhard ;

Jahn, Reinhard ;

Eggeling, Christian ;

Hell, Stefan W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (31) :11440-11445

[4] Effect of fluorescence quenching by stimulated emission on the spectral properties of a solvent-sensitive fluorophore [J].

Gryczynski, I ;

Kusba, J ;

Gryczynski, Z ;

Malak, H ;

Lakowicz, JR .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (24) :10135-10144

[5] Picosecond polarization grating study of the effect of excess excitation energy on the rotational dynamics of rhodamine 6G in different electronic states [J].

Gumy, JC ;

Vauthey, E .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (21) :8628-8632

[6] TEMPERATURE-DEPENDENT MEASUREMENTS OF A FLASHLAMP-PUMPED DYE LASER [J].

HUTH, BG ;

FARMER, GI ;

KAGAN, MR .

JOURNAL OF APPLIED PHYSICS, 1969, 40 (13) :5145-&

[7] Theoretical investigation of the point-spread function given by super-resolving fluorescence microscopy using two-color fluorescence dip spectroscopy [J].

Iketaki, Y ;

Watanabe, T ;

Sakai, M ;

Ishiuchi, S ;

Fujii, M ;

Watanabe, T .

OPTICAL ENGINEERING, 2005, 44 (03) :1-9

[8] Investigation of the fluorescence depletion process in the condensed phase; application to a tryptophan aqueous solution [J].

Iketaki, Y ;

Watanabe, T ;

Ishiuchi, S ;

Sakai, M ;

Omatsu, T ;

Yamamoto, K ;

Fujii, M ;

Watanabe, T .

CHEMICAL PHYSICS LETTERS, 2003, 372 (5-6) :773-778

[9]

IKETAKI Y, 2006, REV SCI INSTRUM, V77

[10] LIGHT QUENCHING OF FLUORESCENCE - A NEW METHOD TO CONTROL THE EXCITED-STATE LIFETIME AND ORIENTATION OF FLUOROPHORES [J].

LAKOWICZ, JR ;

GRYCZYNSKI, I ;

KUSBA, J ;

BOGDANOV, V .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1994, 60 (06) :546-562

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