Changes in temperature modulate heme oxygenase-1 induction by curcumin in renal epithelial cells

被引:45
作者
Balogun, E [1 ]
Foresti, R [1 ]
Green, CJ [1 ]
Motterlini, R [1 ]
机构
[1] Northwick Pk Inst Med Res, Dept Surg Res, Vasc Biol Unit, Harrow HA1 3UJ, Middx, England
关键词
cold storage; tissue preservation; oxidative stress; HO-1;
D O I
10.1016/S0006-291X(03)01517-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The stress protein heme oxygenase-1 (HO-1) plays an essential role in the prevention of transplant-associated organ injury and rejection. Prior to transplantation, organs are normally subjected to variable periods of cold storage in appropriate preservation solutions. Here, we examined whether curcumin, a phenolic plant extract which strongly induces HO-1 in many cell types, could up-regulate HO-1 protein in cultured renal epithelial cells at temperatures lower than the physiological 37degreesC. We found that stimulation of HO-1 following incubation of cells with curcumin for 6 h was dramatically reduced by decreasing the temperature from 37 to 10degreesC. Interestingly, renal cells displayed high HO-1 expression and heme oxygenase activity when exposed to a programmed change in temperature that consisted of 3h at 37degreesC followed by 1.5h at 20degreesC and 1.5h at 10degreesC. Increased HO-1 levels were observed also after incubation of cells with curcumin during the programmed change in temperature under hypoxia, another feature typical of cold storage procedures. Upon challenge with an oxidant-generating system, cells pretreated with curcumin at 37degreesC or during the programmed change in temperature exhibited increased resistance to oxidative stress-mediated injury. These findings highlight the feasibility of modulating HO-1 expression during hypothermic storage to confer tissues a better protection to counteract the damage characteristic of organ transplantation. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码:950 / 955
页数:6
相关论文
共 26 条
[1]  
Agarwal A, 2000, J AM SOC NEPHROL, V11, P965, DOI 10.1681/ASN.V115965
[2]   Upregulation of heme oxygenase-1 protects genetically fat Zucker rat livers from ischemia/reperfusion injury [J].
Amersi, F ;
Buelow, R ;
Kato, H ;
Ke, BB ;
Coito, AJ ;
Shen, XD ;
Zhao, DL ;
Zaky, J ;
Melinek, J ;
Lassman, CR ;
Kolls, JK ;
Alam, J ;
Ritter, T ;
Volk, HD ;
Farmer, DG ;
Ghobrial, RM ;
Busuttil, RW ;
Kupiec-Weglinski, JW .
JOURNAL OF CLINICAL INVESTIGATION, 1999, 104 (11) :1631-1639
[3]   Curcurnin activates the haem oxygenase-1 gene via regulation of Nrf2 and the antioxidant-responsive element [J].
Balogun, E ;
Hoque, M ;
Gong, PF ;
Killeen, E ;
Green, CJ ;
Foresti, R ;
Alam, J ;
Motterlini, R .
BIOCHEMICAL JOURNAL, 2003, 371 :887-895
[4]   Carbon monoxide generated by heme oxygenase 1 suppresses endothelial cell apoptosis [J].
Brouard, S ;
Otterbein, LE ;
Anrather, J ;
Tobiasch, E ;
Bach, FH ;
Choi, AMK ;
Soares, MP .
JOURNAL OF EXPERIMENTAL MEDICINE, 2000, 192 (07) :1015-1025
[5]   Dynamics of haem oxygenase-1 expression and bilirubin production in cellular protection against oxidative stress [J].
Clark, JE ;
Foresti, R ;
Green, CJ ;
Motterlini, R .
BIOCHEMICAL JOURNAL, 2000, 348 (348) :615-619
[6]   Heme oxygenase-1-derived bilirubin ameliorates postischemic myocardial dysfunction [J].
Clark, JE ;
Foresti, R ;
Sarathchandra, P ;
Kaur, H ;
Green, CJ ;
Motterlini, R .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2000, 278 (02) :H643-H651
[7]   Peroxynitrite induces haem oxygenase-1 in vascular endothelial cells: a link to apoptosis [J].
Foresti, R ;
Sarathchandra, P ;
Clark, JE ;
Green, CJ ;
Motterlini, R .
BIOCHEMICAL JOURNAL, 1999, 339 :729-736
[8]   The heme oxygenase pathway and its interaction with nitric oxide in the control of cellular homeostasis [J].
Foresti, R ;
Motterlini, R .
FREE RADICAL RESEARCH, 1999, 31 (06) :459-475
[9]   Thiol compounds interact with nitric oxide in regulating heme oxygenase-1 induction in endothelial cells - Involvement of superoxide and peroxynitrite anions [J].
Foresti, R ;
Clark, JE ;
Green, CJ ;
Motterlini, R .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (29) :18411-18417
[10]   Mechanism of heme oxygenase-1 gene induction by curcumin in human renal proximal tubule cells [J].
Hill-Kapturczak, N ;
Thamilselvan, V ;
Liu, FY ;
Nick, HS ;
Agarwal, A .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2001, 281 (05) :F851-F859