Activation of the Arabidopsis thaliana Mitogen-Activated Protein Kinase MPK11 by the Flagellin-Derived Elicitor Peptide, flg22

被引:108
作者
Bethke, Gerit [1 ,2 ]
Pecher, Pascal [1 ]
Eschen-Lippold, Lennart [1 ]
Tsuda, Kenichi [2 ]
Katagiri, Fumiaki [2 ]
Glazebrook, Jane [2 ]
Scheel, Dierk [1 ]
Lee, Justin [1 ]
机构
[1] Leibniz Inst Plant Biochem Stress & Dev Biol, D-06120 Halle, Germany
[2] Univ Minnesota, Dept Plant Biol, Microbial & Plant Genom Inst, St Paul, MN 55108 USA
基金
美国国家科学基金会;
关键词
INDUCED RESISTANCE; PHYTOALEXIN BIOSYNTHESIS; TRANSCRIPTION FACTOR; DISEASE RESISTANCE; DEFENSE RESPONSES; GENE-EXPRESSION; SALICYLIC-ACID; MAPKS; INTERPLAY; PHOSPHORYLATION;
D O I
10.1094/MPMI-11-11-0281
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mitogen-activated protein kinases (MAPK) mediate cellular signal transduction during stress responses, as well as diverse growth and developmental processes in eukaryotes. Pathogen infection or treatments with conserved pathogen-associated molecular patterns (PAMPs) such as the bacterial flagellin-derived flg22 peptide are known to activate three Arabidopsis thaliana MAPK: MPK3, MPK4, and MPK6. Several stresses, including flg22 treatment, are known to increase MPK11 expression but activation of MPK11 has not been shown. Here, we show that MPK11 activity can, indeed, be increased through flg22 elicitation. A small-scale microarray for profiling defense-related genes revealed that cinnamyl alcohol dehyrogenase 5 requires MPK11 for full flg22-induced expression. An mpk11 mutant showed increased flg22-mediated growth inhibition but no altered susceptibility to Pseudomonas syringae, Botrytis cinerea, or Alternaria brassicicola. In mpk3, mpk6, or mpk4 backgrounds, MPK11 is required for embryo or seed development or general viability. Although this developmental deficiency in double mutants and the lack of or only subtle mpk11 phenotypes suggest functional MAPK redundancies, comparison with the paralogous MPK4 reveals distinct functions. Taken together, future investigations of MAPK roles in stress signaling should include MPK11 as a fourth PAMP-activated MAPK.
引用
收藏
页码:471 / 480
页数:10
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