Conjugation of yeast mannans with protein employing cyanopyridinium agent (CDAP) -: an effective route of antifungal vaccine preparation

被引：17

作者：

Bystricky, S ^{[1
]}

Machová, E ^{[1
]}

Bartek, P ^{[1
]}

Kolarova, N ^{[1
]}

Kogan, G ^{[1
]}

机构：

[1] Slovak Acad Sci, Inst Chem, Bratislava 84238, Slovakia

来源：

GLYCOCONJUGATE JOURNAL | 2000年 / 17卷 / 10期

关键词：

mannan conjugate; galactoglucoxylomannan conjugate; CDAP; antifungal vaccine;

D O I：

10.1023/A:1011002118819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The possibility of using 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) for activation of saccharide hydroxyl groups (instead of hazardous cyanogen bromide) is examined with cell-surface mannans of the yeasts Candida albicans,Candida tropicalis, Candida lambica and galactoglucoxylomannan of Cryptococcus laurentii. Direct conjugation with human serum albumin yielded soluble products with increased molecular size in comparison with the original polysaccharides. Immunodiffusion experiments revealed that conjugation did not affect the immunospecificity of the antigen epitope.

引用

页码：677 / 680

页数：4

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