A simple technique for direct transformation and regeneration of the diploid legume species Lotus japonicus

被引：14

作者：

Oger, P ^{[1
]}

Petit, A ^{[1
]}

Dessaux, Y ^{[1
]}

机构：

[1] CNRS,INST SCI VEGETALES,F-91198 GIF SUR YVETTE,FRANCE

来源：

PLANT SCIENCE | 1996年 / 116卷 / 02期

关键词：

Lotus japonicus; Agrobacterium; legume transformation; regeneration;

D O I：

10.1016/0168-9452(96)04391-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The diploid legume species Lotus japonicus was transformed using a disarmed Agrobacterium strain. By inoculating the cotyledon attachment site, we took advantage of the regenerative capability of the plant which spontaneously regenerated transformed shoots. Some of these shoots were propagated as transgenic plants and later transferred to the glasshouse to produce seeds. Seeds were germinated and yielded non-chimeric, transgenic stably modified Lotus plants.

引用

页码：159 / 168

页数：10

共 20 条

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