Characterisation of hexon and fibre genes of a novel strain of adenovirus involved in epidemic keratoconjunctivitis

被引：10

作者：

Adhikary, AK

Inada, T

Numaga, J

Suzuki, E

Ushijima, H

Banik, U

Mukouyama, A

Matsuno, S

Okabe, N

机构：

[1] Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Shinjuku Ku, Tokyo 1628640, Japan

[2] Univ Tokyo, Grad Sch Med, Dept Ophthalmol, Bunkyo Ku, Tokyo 1130033, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Dev Med Sci, Tokyo 1130033, Japan

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2004年 / 57卷 / 01期

关键词：

D O I：

10.1136/jcp.57.1.95

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Aims: To characterise a novel strain (M86) of adenovirus ( Ad) involved in epidemic keratoconjunctivitis (EKC). Methods/Results: The virus strain was neutralised by antisera to both Ad35 and Ad11. Restriction endonuclease analysis of genomic DNA showed 98% and 88% homology with Ad11 and Ad35, respectively. The deduced amino acid sequence of the hypervariable regions of (HVRs) of the hexon gene showed a higher homology with Ad35 (94.4%) than with Ad11 (83.7%). However, it was 100% homologous to Ad35 in HVRs 1, 2, 3, and 6 and to Ad11 in HVRs 4 and 6. In the fibre knob, the isolate was more homologous to Ad11 (99.4%) than to Ad35 (29.1%). Conclusion: This novel strain of adenovirus showed similarities with both Ad11 and Ad35. The isolation of a novel strain like Ad35+11 is important because of its association with EKC.

引用

页码：95 / 97

页数：3