Synthesis of reaper, a cysteine-containing polypeptide, using a peptide thioester in the presence of silver chloride as an activator

被引：22

作者：

Kawakami, T ^{[1
]}

Yoshimura, S ^{[1
]}

Aimoto, S ^{[1
]}

机构：

[1] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan

来源：

TETRAHEDRON LETTERS | 1998年 / 39卷 / 43期

关键词：

peptides and polypeptides; thioesters; silver and compounds; condensations;

D O I：

10.1016/S0040-4039(98)01752-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Reaper was synthesized by condensing two peptide segments, one of which was a partially protected peptide thioester, Boc-[Lys(Boc)(20)]-reaper( 1-32)-SCH2CH2CO-beta-Ala-NH2, and the other a peptide which contained an Cys(Acm) residue, H-[Cys(Acm)(49), Lys(Boc)(52,56,59,62)]-reaper(33-65)-OH. The condensation proceeded without loss of the Acm group when silver chloride was used as an activator of thioester moiety, This result indicates that silver chloride is a generally applicable activating reagent to the preparation of polypeptides which contain Cys(Acm) residues. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：7901 / 7904

页数：4

共 5 条

[1] POLYPEPTIDE-SYNTHESIS USING THE S-ALKYL THIOESTER OF A PARTIALLY PROTECTED PEPTIDE SEGMENT - SYNTHESIS OF THE DNA-BINDING DOMAIN OF C-MYB PROTEIN (142-193)-NH2 [J].