Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability

被引：65

作者：

Zhu, Linjiang ^{[1
,2
]}

Dong, Hongjun ^{[1
,2
]}

Zhang, Yanping ^{[1
]}

Li, Yin ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

来源：

METABOLIC ENGINEERING | 2011年 / 13卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Clostridium acetobutylicum; Robustness; Glutathione; Aero-tolerance; Butanol tolerance; Butanol production; GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; PROTECTS LACTOCOCCUS-LACTIS; ESCHERICHIA-COLI; OXIDATIVE-STRESS; TRANSCRIPTIONAL PROGRAM; SOLVENT PRODUCTION; OSMOTIC-STRESS; TOLERANCE; BUTANOL; BACTERIA;

D O I：

10.1016/j.ymben.2011.01.009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To improve the aero- and solvent tolerance of the solvent-producing Clostridium acetobutylicum, glutathione biosynthetic capability was introduced into C acetobutylicum DSM1731 by cloning and over-expressing the gshAB genes from Escherichia coli. Strain DSM1731(pITAB) produces glutathione, and shows a significantly improved survival upon aeration and butanol challenge, as compared with the control. In addition, strain DSM1731(pITAB) exhibited an improved butanol tolerance and an increased butanol production capability, as compared with the recombinant strains with only gshA or gshB gene. These results illustrated that introducing glutathione biosynthetic pathway, which is redundant for the metabolism of C acetobutylicum, can increase the robustness of the host to achieve a better solvent production. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：426 / 434

页数：9

共 59 条

[1] Metabolite Stress and Tolerance in the Production of Biofuels and Chemicals: Gene-Expression-Based Systems Analysis of Butanol, Butyrate, and Acetate Stresses in the Anaerobe Clostridium acetobutylicum [J].

Alsaker, Keith V. ;

Paredes, Carlos ;

Papoutsakis, Eleftherios T. .

BIOTECHNOLOGY AND BIOENGINEERING, 2010, 105 (06) :1131-1147

[2] Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum [J].

Alsaker, KV ;

Papoutsakis, ET .

JOURNAL OF BACTERIOLOGY, 2005, 187 (20) :7103-7118

[3]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[4] Metabolic engineering of Escherichia coli for 1-butanol production [J].

Atsumi, Shota ;

Cann, Anthony F. ;

Connor, Michael R. ;

Shen, Claire R. ;

Smith, Kevin M. ;

Brynildsen, Mark P. ;

Chou, Katherine J. Y. ;

Hanai, Taizo ;

Liao, James C. .

METABOLIC ENGINEERING, 2008, 10 (06) :305-311

[5] Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels [J].

Atsumi, Shota ;

Hanai, Taizo ;

Liao, James C. .

NATURE, 2008, 451 (7174) :86-U13

[6] Dynamics of genomic-library enrichment and identification of solvent tolerance genes for Clostfidium acetobutylicum [J].

Borden, Jacob R. ;

Papoutsakis, Eleftherios Terry .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (09) :3061-3068

[7] A genomic-library based discovery of a novel, possibly synthetic, acid-tolerance mechanism in Clostridium acetobutylicum involving non-coding RNAs and ribosomal RNA processing [J].

Borden, Jacob R. ;

Jones, Shawn W. ;

Indurthi, Dinesh ;

Chen, Yili ;

Papoutsakis, Eleftherios Terry .

METABOLIC ENGINEERING, 2010, 12 (03) :268-281

[8] EFFECTS OF BUTANOL ON CLOSTRIDIUM-ACETOBUTYLICUM [J].

BOWLES, LK ;

ELLEFSON, WL .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 50 (05) :1165-1170

[9] S-glutathionylation of glyceraldehyde-3-phosphate dehydrogenase: Role of thiol oxidation and catalysis by glutaredoxin [J].

Cotgreave, IA ;

Gerdes, R ;

Schuppe-Koistinen, I ;

Lind, C .

PROTEIN SENSORS AND REACTIVE OXYGEN SPECIES, PT B, THIOL ENZYMES AND PROTEINS, 2002, 348 :175-182

[10] PHYSIOLOGICAL AND GENETIC RESPONSES OF BACTERIA TO OSMOTIC-STRESS [J].

CSONKA, LN .

MICROBIOLOGICAL REVIEWS, 1989, 53 (01) :121-147

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